Journal ArticleDOI
Fourier ring correlation as a resolution criterion for super-resolution microscopy.
TLDR
It is shown that Fourier ring correlation provides an easy-to-use, laboratory consistent standard for measuring the resolution of SRM images, and is provided a freely available software tool that combines resolution measurement with image reconstruction.About:
This article is published in Journal of Structural Biology.The article was published on 2013-09-01. It has received 288 citations till now. The article focuses on the topics: Resolution (electron density) & Near-field scanning optical microscope.read more
Citations
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Journal ArticleDOI
Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations.
Nils Gustafsson,Siân Culley,George W. Ashdown,Dylan M. Owen,Pedro M. Pereira,Ricardo Henriques +5 more
TL;DR: The broad applicability of SRRF and its performance at low signal-to-noise ratios allows super-resolution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude lower than methods such as PALM, STORM or STED.
Journal ArticleDOI
Precisely and accurately localizing single emitters in fluorescence microscopy
Hendrik Deschout,Francesca Cella Zanacchi,Michael J. Mlodzianoski,Alberto Diaspro,Joerg Bewersdorf,Samuel T. Hess,Kevin Braeckmans +6 more
TL;DR: A lucid synthesis of the developments on single-molecule localization precision and accuracy and their practical implications are presented in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy.
Journal ArticleDOI
Quantitative evaluation of software packages for single-molecule localization microscopy
Daniel Sage,Hagai Kirshner,Thomas Pengo,Nico Stuurman,Junhong Min,Suliana Manley,Michael Unser +6 more
TL;DR: This work focuses on the computational aspects of super-resolution microscopy and presents a comprehensive evaluation of localization software packages, reflecting the various tradeoffs of SMLM software packages and helping users to choose the software that fits their needs.
Journal ArticleDOI
Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking
TL;DR: In this article, a variety of methods for obtaining both 3D super-resolution images and 3D tracking infers structures or motions extending in the axial direction can easily be missed or confused.
Journal ArticleDOI
α-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson's disease.
Marthe H.R. Ludtmann,Marthe H.R. Ludtmann,Plamena R. Angelova,Mathew H. Horrocks,Mathew H. Horrocks,Minee L. Choi,Minee L. Choi,Margarida Rodrigues,Artyom Y. Baev,A. V. Berezhnov,Zhi Yao,Zhi Yao,Daniel Little,Blerida Banushi,Afnan Saleh Al-Menhali,Rohan T. Ranasinghe,Daniel R. Whiten,Ratsuda Yapom,Karamjit Singh Dolt,Michael J. Devine,Paul Gissen,Tilo Kunath,Morana Jaganjac,Evgeny Pavlov,David Klenerman,Andrey Y. Abramov,Sonia Gandhi,Sonia Gandhi +27 more
TL;DR: It is shown that specific oligomers of α-synuclein exert effects on mitochondria to induce opening of the permeability transition pore, leading to cell death in Parkinson’s disease.
References
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Journal ArticleDOI
Imaging intracellular fluorescent proteins at nanometer resolution.
Eric Betzig,George H. Patterson,Rachid Sougrat,O. Wolf Lindwasser,Scott G. Olenych,Juan S. Bonifacino,Michael W. Davidson,Jennifer Lippincott-Schwartz,Harald F. Hess +8 more
TL;DR: This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.
Journal ArticleDOI
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
TL;DR: A high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores that can, in principle, reach molecular-scale resolution is developed.
Journal ArticleDOI
Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
TL;DR: A new method for fluorescence imaging has been developed that can obtain spatial distributions of large numbers of fluorescent molecules on length scales shorter than the classical diffraction limit, and suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution.
Journal ArticleDOI
Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes†
Mike Heilemann,Sebastian van de Linde,Mark Schüttpelz,Robert Kasper,Britta Seefeldt,Anindita Mukherjee,Philip Tinnefeld,Markus Sauer +7 more
Journal ArticleDOI
The correlation averaging of a regularly arranged bacterial cell envelope protein
W. O. Saxton,Wolfgang Baumeister +1 more
TL;DR: An adaptation of the ‘correlation averaging’ method is described which allows reliable and almost fully automatic image averaging in the case of near‐periodic structures notwithstanding the presence of substantial crystal imperfections.