Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae.
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TLDR
It is demonstrated that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recomb inase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.Abstract:
The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.read more
Citations
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A New Efficient Gene Disruption Cassette for Repeated Use in Budding Yeast
TL;DR: A loxP-kanMX-loxP gene disruption cassette is developed, which combines the advantages of the heterologous kanr marker with those from the Cre- lox P recombination system, and will be of great advantage for the functional analysis of gene families.
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Intestinal polyposis in mice with a dominant stable mutation of the β-catenin gene
Naomoto Harada,Yoshitaka Tamai,Tomo O. Ishikawa,Brian C. Sauer,Brian C. Sauer,Kazuaki Takaku,Masanobu Oshima,Makoto Mark Taketo +7 more
TL;DR: Experimental genetic evidence is presented that activation of the Wnt signaling pathway can cause intestinal and colonic tumors.
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Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting.
TL;DR: This work has employed a method based on the Cre-loxP recombination system of bacteriophage P1 to generate a mouse strain in which the JH segments and the intron enhancer in the IgH locus are deleted, and shows that, on the mutant chromosome, switch recombination at the mu gene switch region is strongly suppressed, whereas the switch region of the gamma 1 gene is efficiently rearranged.
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The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome.
Kent G. Golic,Susan Lindquist +1 more
TL;DR: The site-specific recombination system of the yeast 2 micron plasmid, the FLP recombinase and its recombination targets (FRTs), into the genome of Drosophila, producing white-eyed and dark-red-eyed progeny.
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Construction of adenovirus vectors through Cre-lox recombination.
TL;DR: First, a simple method for constructing E1-substituted adenovirus vectors carrying recombinant genes in place of all of the viral genes is demonstrated, so-called gutless adenOVirus vectors.
References
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TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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