Functional genetics of human gut commensal Bacteroides thetaiotaomicron reveals metabolic requirements for growth across environments
Hualan Liu,Anthony L. Shiver,Morgan N. Price,Hans K. Carlson,Valentine V. Trotter,Yan Chen,Veronica Escalante,Jayashree Ray,Kelsey E. Hern,Christopher J. Petzold,Peter J. Turnbaugh,Kerwyn Casey Huang,Adam P. Arkin,Adam P. Arkin,Adam M. Deutschbauer,Adam M. Deutschbauer +15 more
TLDR
In this article, the authors measured the growth of a barcoded transposon mutant library of the gut commensal Bacteroides thetaiotaomicron on 48 carbon sources, in the presence of 56 stress-inducing compounds, and during mono-colonization of gnotobiotic mice.About:
This article is published in Cell Reports.The article was published on 2021-03-02 and is currently open access. It has received 50 citations till now. The article focuses on the topics: Bacteroides thetaiotaomicron.read more
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<i>Bacteroides thetaiotaomicron</i> uses a widespread extracellular DNase to promote bile-dependent biofilm formation
TL;DR: In this article , the authors studied biofilm formation in Bacteroides thetaiotaomicron , an anaerobic gut symbiont degrading diet sugars and contributing to gut maturation.
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Ruminococcus bromii enables the growth of proximal Bacteroides thetaiotaomicron by releasing glucose during starch degradation.
Aathmaja Anandhi Rangarajan,Hannah E Chia,Christopher A. Azaldegui,Monica Olszewski,Gabriel V. Pereira,Nicole M. Koropatkin,Julie S. Biteen +6 more
TL;DR: The results indicate that starch degradation by Rb cross-feeds other bacteria in the surrounding region by releasing glucose, and elucidate the adaptive morphology of Bt cells under different nutrient and physiological conditions.
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M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform
Po-Hsun Huang,Sijie Chen,Anthony L. Shiver,Rebecca N Culver,Kerwyn Casey Huang,Cullen R. Buie +5 more
TL;DR: This work presents a scalable, large-scale bacterial gene delivery approach enabled by a disposable, user-friendly microfluidic electroporation device requiring minimal device fabrication and straightforward operation and demonstrates that the proposed device can outperform conventional cuvettes in a range of situations.
Journal ArticleDOI
Genome-Wide Identification of Genes Important for Growth of Dickeya dadantii and Dickeya dianthicola in Potato (Solanum tuberosum) Tubers
TL;DR: Though the Soft Rot Pectobacteriaceae can cause disease with little host specificity, it remains to be seen the extent to which strain-level variation impacts virulence.
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Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron
Odis Johnson,Zhengguo Sheng +1 more
TL;DR: In this article , the assembly of a genome-scale ordered collection of transposon-insertion mutants in the model gut anaerobe Bacteroides thetaiotaomicron VPI-5482 is described.
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