Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells
Veli-Jukka Uitto,Veli-Jukka Uitto,Daniel Baillie,Qiang Wu,Renée Gendron,Daniel Grenier,Edward E. Putnins,Arja Kanervo,James D. Firth +8 more
TLDR
The study suggests that F. nucleatum may be involved in the pathogenesis of periodontal diseases by activating multiple cell signaling systems that lead to stimulation of collagenase 3 expression and increased migration and survival of the infected epithelial cells.Abstract:
Fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. Initiation and outcome of infective diseases depend critically on the host cell signaling system altered by the microbe. Production of proteinases by infected cells is an important factor in pericellular tissue destruction and cell migration. We studied binding of F. nucleatum to human epithelial cells (HaCaT keratinocyte line) and subsequent cell signaling related to collagenase 3 expression, cell motility, and cell survival, using a scratch wound cell culture model. F. nucleatum increased levels of 12 protein kinases involved in cell migration, proliferation, and cell survival signaling, as assessed by the Kinetworks immunoblotting system. Epithelial cells of the artificial wound margins were clearly preferential targets of F. nucleatum. The bacterium colocalized with lysosomal structures and stimulated migration of these cells. Of the 13 anaerobic oral bacterial species, F. nucleatum and Fusobacterium necrophorum were among the best inducers of collagenase 3 mRNA levels, a powerful matrix metalloproteinase. Production of collagenase 3 was detected in fusobacterium-infected cells and cell culture medium by immunocytochemistry, immunoblotting, and zymography. The proteinase production involved activation of p38 mitogen-activated protein kinase in the infected cells. The study suggests that F. nucleatum may be involved in the pathogenesis of periodontal diseases (and other infections) by activating multiple cell signaling systems that lead to stimulation of collagenase 3 expression and increased migration and survival of the infected epithelial cells.read more
Citations
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Genomic analysis identifies association of Fusobacterium with colorectal carcinoma
Aleksandar Kostic,Dirk Gevers,Chandra Sekhar Pedamallu,Chandra Sekhar Pedamallu,Monia Michaud,Fujiko Duke,Fujiko Duke,Ashlee M. Earl,Akinyemi I. Ojesina,Akinyemi I. Ojesina,Joonil Jung,Adam J. Bass,Adam J. Bass,Josep Tabernero,José Baselga,Chen Liu,Ramesh A. Shivdasani,Shuji Ogino,Bruce W. Birren,Curtis Huttenhower,Curtis Huttenhower,Wendy S. Garrett,Wendy S. Garrett,Matthew Meyerson,Matthew Meyerson +24 more
TL;DR: The composition of the microbiota in colorectal carcinoma is characterized using whole genome sequences from nine tumor/normal pairs and Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs.
Journal ArticleDOI
Invasive potential of gut mucosa-derived Fusobacterium nucleatum positively correlates with IBD status of the host.
Jaclyn Strauss,Gilaad G. Kaplan,Paul L. Beck,Kevin P. Rioux,Remo Panaccione,Rebekah DeVinney,Tarah Lynch,Emma Allen-Vercoe +7 more
TL;DR: This study indicates that colonization of the intestinal mucosa by highly invasive strains of F. nucleatum may be a useful biomarker for gastrointestinal disease.
Journal ArticleDOI
Microbiota disbiosis is associated with colorectal cancer.
TL;DR: It is suggested that the mucosa-associated microbiota is dynamically associated with CRC, which may provide evidences for microbiota-associated diagnostic, prognostic, preventive, and therapeutic strategies for CRC.
Journal ArticleDOI
Fusobacterium in colonic flora and molecular features of colorectal carcinoma
Tomomitsu Tahara,Tomomitsu Tahara,Eiichiro Yamamoto,Hiromu Suzuki,Reo Maruyama,Woonbok Chung,Judith Garriga,Jaroslav Jelinek,Hiro O. Yamano,Tamotsu Sugai,Byonggu An,Imad Shureiqi,Minoru Toyota,Yutaka Kondo,Marcos R. Estecio,Jean Pierre J. Issa +15 more
TL;DR: It is shown that Fusobacterium enrichment is associated with specific molecular subsets of colorectal cancers, offering support for a pathogenic role in coloreCTal cancer for this gut microbiome component.
Journal ArticleDOI
Fusobacterium nucleatum in periodontal health and disease.
TL;DR: This review will focus on expression, function, regulation and functional efficacy of antimicrobial peptides against F. nucleatum, one of the most interesting gram-negative bacteria implicated in periodontal diseases.
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