Journal ArticleDOI
GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues
Reads0
Chats0
TLDR
These data provide an extensive analysis of GAPDH mRNA expression in human tissues and confirm previous reports of the marked variability ofGAPDH expression between tissue types.Abstract:
Quantitative gene expression data are often normalized to the expression levels of control or so-called “housekeeping” genes. An inherent assumption in the use of housekeeping genes is that express...read more
Citations
More filters
Journal ArticleDOI
Human housekeeping genes, revisited.
Eli Eisenberg,Erez Y. Levanon +1 more
TL;DR: This work describes housekeeping gene detection in the era of massive parallel sequencing and RNA-seq and provides a list of 3804 human genes that are expressed uniformly across a panel of tissues.
Journal ArticleDOI
Reference genes in real-time PCR.
Bartłomiej Kozera,Marcin Rapacz +1 more
TL;DR: Various aspects of the use of reference genes in qPCR technique used in the thousands of present studies are discussed, including examples of best normalizing genes in some specific cases and possible mistakes are presented based on available sources.
Journal ArticleDOI
Selection of reliable reference genes for gene expression studies in peach using real-time PCR
TL;DR: This study demonstrates that expression stability varied greatly between genes studied in peach, and TEF2, UBQ10 and RP II were found to be the most suitable reference genes with a very high statistical reliability, while 18S rRNA, RPL13 and PLA2 were unsuitable as internal controls.
Journal ArticleDOI
Babesiosis: recent insights into an ancient disease.
TL;DR: Recent insights into human babesiosis with regard to phylogeny, diagnostics and treatment are covered in order to provide new information on well known as well as recently discovered parasites with zoonotic potential.
Journal ArticleDOI
Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions
TL;DR: Gapdh is the recommended reference gene for measuring gene expression in Coffea arabica and will enable more accurate and reliable normalization of tissue/organ-specific gene expression studies in this important cherry crop plant.
References
More filters
Journal ArticleDOI
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Jo Vandesompele,Katleen De Preter,Filip Pattyn,Bruce Poppe,Nadine Van Roy,Anne De Paepe,Franki Speleman +6 more
TL;DR: The normalization strategy presented here is a prerequisite for accurate RT-PCR expression profiling, which opens up the possibility of studying the biological relevance of small expression differences.
Journal ArticleDOI
Housekeeping genes as internal standards: use and limits.
Olivier Thellin,Willy Zorzi,Bernard Lakaye,B. de Borman,Bernard Coumans,Georges Hennen,Thierry Grisar,Ahmed Igout,Ernst Heinen +8 more
TL;DR: It is recalled that the commonly used internal standards can quantitatively vary in response to various factors and possible variations are illustrated using three experimental examples.
Journal ArticleDOI
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
Keertan Dheda,Jim F. Huggett,Stephen A. Bustin,Margaret A. Johnson,Graham A. W. Rook,Alimuddin Zumla +5 more
TL;DR: Real-time reverse transcription PCR is used to assess the levels of 13 housekeeping genes expressed in peripheral blood mononuclear cell culture and whole blood from healthy individuals and those with tuberculosis and shows that RNA specifying human acidic ribosomal protein was the most suitable housekeeping gene for normalizing mRNA levels in human pulmonary tuberculosis.
Journal ArticleDOI
Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies ☆
Carmela Tricarico,Pamela Pinzani,Simonetta Bianchi,Milena Paglierani,Vito Distante,Mario Pazzagli,Stephen A. Bustin,Claudio Orlando +7 more
TL;DR: The results suggest that the use of internal standards comprising single housekeeping genes or rRNA is inappropriate for studies involving tissue biopsies.
Journal ArticleDOI
Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes
TL;DR: Gene expression levels of about 7,000 genes were measured in 11 different human adult and fetal tissues using high-density oligonucleotide arrays to identify genes involved in cellular maintenance in normal individuals.