Journal ArticleDOI
Geraniol hydroxylase and hydroxygeraniol oxidase activities of the CYP76 family of cytochrome P450 enzymes and potential for engineering the early steps of the (seco)iridoid pathway.
René Höfer,Lemeng Dong,François André,Jean-François Ginglinger,Raphaël Lugan,Carole Gavira,Sébastien Grec,Gerhard Lang,Johan Memelink,Sander van der Krol,Harro J. Bouwmeester,Danièle Werck-Reichhart +11 more
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TLDR
An extended functional analysis of CYP76 family members was carried-out to identify the most effective enzyme to be used for pathway reconstruction, and CyP76B6 emerged as a highly specialized multifunctional enzyme catalyzing two sequential oxidation steps leading to the formation of 8-oxogeraniol from geraniol.About:
This article is published in Metabolic Engineering.The article was published on 2013-11-01. It has received 86 citations till now. The article focuses on the topics: Geraniol & Hydroxylation.read more
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The seco-iridoid pathway from Catharanthus roseus
Karel Miettinen,Lemeng Dong,Nicolas Navrot,Thomas Schneider,Vincent Burlat,Jacob Pollier,Lotte S. Woittiez,Sander van der Krol,Raphaël Lugan,Tina Ilc,Robert Verpoorte,Kirsi-Marja Oksman-Caldentey,Enrico Martinoia,Harro J. Bouwmeester,Alain Goossens,Johan Memelink,Danièle Werck-Reichhart +16 more
TL;DR: expression of the eight genes encoding this pathway, together with two genes boosting precursor formation and two downstream alkaloid biosynthesis genes, in an alternative plant host allows the heterologous production of the complex MIA strictosidine, which confirms the functionality of all enzymes of the pathway and highlights their utility for synthetic biology programmes.
Journal ArticleDOI
Cytochrome P450-mediated metabolic engineering: current progress and future challenges.
Hugues Renault,Jean-Etienne Bassard,Björn Hamberger,Danièle Werck-Reichhart,Danièle Werck-Reichhart +4 more
TL;DR: Hurdles encountered in P450 engineering and their potential solutions are summarized here and lessons from this pioneering work should considerably boost exploitation of the amazing P450 toolkit emerging from accelerated sequencing of plant genomes.
Journal ArticleDOI
Expanding P450 catalytic reaction space through evolution and engineering.
TL;DR: The cytochrome P450 enzyme family, whose members participate in xenobiotic metabolism and natural products biosynthesis, catalyzes an impressive range of difficult chemical reactions that continues to grow as new enzymes are characterized.
Journal ArticleDOI
Engineering of Secondary Metabolism.
TL;DR: A critical overview of engineering approaches in secondary metabolism is presented, both in heterologous and native hosts, and the recognition of the increasing role of compartmentalization in metabolic engineering is highlighted.
Journal ArticleDOI
Gene Coexpression Analysis Reveals Complex Metabolism of the Monoterpene Alcohol Linalool in Arabidopsis Flowers
Jean-François Ginglinger,Benoît Boachon,René Höfer,Christian Paetz,Tobias G. Köllner,Laurence Miesch,Raphaël Lugan,Raymonde Baltenweck,Jérôme Mutterer,Pascaline Ullmann,Franziska Beran,Patricia Claudel,Francel W.A. Verstappen,Marc J. C. Fischer,Francis Karst,Harro J. Bouwmeester,Michel Miesch,Bernd Schneider,Jonathan Gershenzon,Jürgen Ehlting,Danièle Werck-Reichhart +20 more
TL;DR: This work characterizes two cytochrome P450s and two monoterpene synthases that are coexpressed in flowers and thus predicted to be involved in monoterpenoid metabolism.
References
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Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Book
Cytochrome P-450: Structure, Mechanism, and Biochemistry
Ortiz de Montellano,R Paul +1 more
TL;DR: The Cytochrome P450 and the Metabolism and Bioactivation of Arachidonic Acid and Eicosanoids in Plants and the Diversity and Importance of Microbial Cytochromes P450 are studied.
Journal ArticleDOI
High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
TL;DR: A high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae is described, which gives the highest efficiency and yield of transformants.
Journal ArticleDOI
An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.
TL;DR: A system based on co-expression of a viral-encoded suppressor of gene silencing, the p19 protein of tomato bushy stunt virus, that prevents the onset of PTGS in the infiltrated tissues and allows high level of transient expression is described.
Journal ArticleDOI
Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry
TL;DR: A detailed protocol for large-scale untargeted metabolomics of plant tissues, based on reversed phase liquid chromatography coupled to high-resolution mass spectrometry (LC-QTOF MS) of aqueous methanol extracts is described.