Journal ArticleDOI
H2S is involved in ABA-mediated stomatal movement through MPK4 to alleviate drought stress in Arabidopsis thaliana
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TLDR
In this article, the relationship between H2S and MAPK in drought stress resistance in Arabidopsis was explored, and the role of H2O2 and MPK4 in stomatal movements in response to abscisic acid (ABA) and hydrogen peroxide (H 2O2) was examined.Abstract:
Hydrogen sulfide (H2S) is a gaseous signaling molecule that participates in multiple physiological processes in both animals and plants. Mitogen-activated protein kinase (MAPK) is important signaling molecule that links the growth and developmental signals and environment stimuli to cellular responses. In the current study we explored the relationship between H2S and MAPK in drought stress resistance in Arabidopsis. The quantitative real-time (qRT)-PCR, root tip bending experiment and stomatal aperture assay were used in this paper. Drought stress activated both H2S biosynthesis and gene expression of MAPKs. The increase in MAPK expression was depressed in lcd/des1, a double mutant of H2S synthesis. Then we selected MPK4 as our target and used mpk4 mutants for further studies. H2S was able to alleviate the drought stress in wild-type (WT) Arabidopsis but not in mpk4 mutants. Meanwhile, H2S-induced stomatal movement was impaired in mpk4 mutants. We then examined the role of H2S and MPK4 in stomatal movements in response to abscisic acid (ABA) and hydrogen peroxide (H2O2). ABA- and H2O2- mediated stomatal movements were impaired in lcd/des1 and mpk4 mutants, and H2S-induced stomatal closure was impaired in slac1–3 mutants. Our results suggested that MPK4 is important downstream of H2S in the drought stress response and in stomatal movement, and that the H2S-MPK4 cascade is involved in ABA-mediated stomatal movement to regulate the drought stress.read more
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Journal ArticleDOI
Persulfidation-based Modification of Cysteine Desulfhydrase and the NADPH Oxidase RBOHD Controls Guard Cell Abscisic Acid Signaling.
Jie Shen,Jing Zhang,Mingjian Zhou,Heng Zhou,Beimi Cui,Cecilia Gotor,Luis C. Romero,Ling Fu,Jing Yang,Christine H. Foyer,Christine H. Foyer,Qiaona Pan,Qiaona Pan,Wenbiao Shen,Yanjie Xie +14 more
TL;DR: It is shown that ABA stimulates the persulfidation of l-CYSTEINE DESULFHYDRASE1, an important endogenous H2S enzyme, at Cys44 and Cys205 in a redox-dependent manner, and s-persulfidation-induced RBOHD activity is relevant to ABA-induced stomatal closure.
Journal ArticleDOI
Hydrogen sulfide, a signaling molecule in plant stress responses
Jing Zhang,Mingjian Zhou,Heng Zhou,Didi Zhao,Cecilia Gotor,Luis C. Romero,Jie Shen,Zhenglin Ge,Zhirong Zhang,Wenbiao Shen,Xingxing Yuan,Yanjie Xie +11 more
TL;DR: This review highlight what the authors currently know about the characteristics and biosynthesis of H2 S in plants and propose and discuss possible redox-dependent mechanisms by which H2S regulates plant physiology.
Journal ArticleDOI
Abscisic acid-triggered guard cell l-cysteine desulfhydrase function and in situ hydrogen sulfide production contributes to heme oxygenase-modulated stomatal closure.
Jing Zhang,Mingjian Zhou,Zhenglin Ge,Jie Shen,Can Zhou,Cecilia Gotor,Luis C. Romero,Xingliang Duan,Xin Liu,Wu Deliang,Xianchao Yin,Yanjie Xie +11 more
TL;DR: It is indicated that guard cell-in situ DES1 function is involved in ABA-induced stomatal closure, which also acts as a pivotal hub in regulating HY1 signaling.
Journal ArticleDOI
Hydrogen sulfide-linked persulfidation of ABI4 controls ABA responses through the transactivation of MAPKKK18 in Arabidopsis.
Mingjian Zhou,Jing Zhang,Jing Zhang,Jie Shen,Heng Zhou,Didi Zhao,Cecilia Gotor,Luis C. Romero,Ling Fu,Zongmin Li,Jing Yang,Wenbiao Shen,Xingxing Yuan,Yanjie Xie +13 more
TL;DR: In this article, the authors showed that ABSCISIC ACID INSENSITIVE 4 (ABI4) acts downstream of DES1 in the control of ABA responses in Arabidopsis.
Journal ArticleDOI
The role of hydrogen sulfide in plant alleviates heavy metal stress
Shilei Luo,Alejandro Calderón-Urrea,Alejandro Calderón-Urrea,Jihua Yu,Weibiao Liao,Jianming Xie,Jian Lv,Zhi Feng,Zhongqi Tang +8 more
TL;DR: In this paper, the authors explored the role of hydrogen sulfide (H2S) as a plant-signaling molecule, which participates in the response to heavy metal stress and enhances plant tolerance to various stresses.
References
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Journal ArticleDOI
The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener
TL;DR: It is demonstrated that H2S is an important endogenous vasoactive factor and the first identified gaseous opener of KATP channels in vascular SMCs and production from vascular tissues was enhanced by nitric oxide.
Journal ArticleDOI
Mitogen-activated protein kinase cascades in plants: a new nomenclature
Kazuya Ichimura,Kazuo Shinozaki,Guillaume Tena,Jen Sheen,Yves Henry,Anthony Champion,Martin Kreis,Shuqun Zhang,Heribert Hirt,Cathal Wilson,Erwin Heberle-Bors,Brian E. Ellis,Peter Morris,Roger W. Innes,Joseph R. Ecker,Dierk Scheel,Daniel F. Klessig,Yasunori Machida,John Mundy,Yuko Ohashi,John C. Walker +20 more
TL;DR: A simplified nomenclature for Arabidopsis MAPKs and MAPK kinases is proposed that might also serve as a basis for standard annotation of these gene families in all plants.
Journal ArticleDOI
Arabidopsis MAP Kinase 4 Negatively Regulates Systemic Acquired Resistance
Morten Petersen,Peter Brodersen,Henrik Næsted,Erik Andreasson,Ursula Lindhart,Bo Johansen,Henrik Nielsen,Michelle Lacy,Mark J. Austin,Jane E. Parker,Sashi B. Sharma,Daniel F. Klessig,Robert A. Martienssen,Ole Mattsson,Anders Boeck Jensen,John Mundy +15 more
TL;DR: Transposon inactivation of Arabidopsis MAP kinase 4 produced the mpk4 mutant exhibiting constitutive systemic acquired resistance (SAR) including elevated salicylic acid levels, increased resistance to virulent pathogens, and constitutive pathogenesis-related gene expression shown by Northern and microarray hybridizations.
Journal ArticleDOI
Mitogen-Activated Protein Kinase Signaling in Plants
TL;DR: Genetic analyses have uncovered several predominant MAPK components shared by several of these processes including the Arabidopsis thaliana MAPKs MPK3, 4, and 6 and MAP2Ks MKK1, 2, 3, and 5, and future work needs to focus on identifying substrates ofMAPKs, and on understanding how specificity is achieved among MAPK signaling pathways.
Journal ArticleDOI
SLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling
Triin Vahisalu,Hannes Kollist,Hannes Kollist,Yong-Fei Wang,Noriyuki Nishimura,Wai-Yin Chan,Gabriel Valerio,Airi Lamminmäki,Mikael Brosché,Heino Moldau,Radhika Desikan,Radhika Desikan,Julian I. Schroeder,Jaakko Kangasjärvi +13 more
TL;DR: The mapping and characterization of an ozone-sensitive Arabidopsis thaliana mutant, slac1, is reported, showing that SLAC1 is preferentially expressed in guard cells and encodes a distant homologue of fungal and bacterial dicarboxylate/malic acid transport proteins, and a vital role forSLAC1 in the function of S-type anion channels is suggested.