Journal ArticleDOI
Heterologous protection against influenza by injection of DNA encoding a viral protein
Jeffrey B. Ulmer,John J. Donnelly,Suezanne E. Parker,Gary Rhodes,Philip L. Felgner,V. J. Dwarki,Stanislaw H. Gromkowski,R. Randall Deck,Corrille M. DeWitt,Arthur Friedman,Linda A. Hawe,Karen R. Leander,Douglas Martinez,Helen C. Perry,John W. Shiver,Donna L. Montgomery,Margaret A. Liu +16 more
TLDR
To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleop protein was injected into the quadriceps of BALB/c mice and resulted in the generation of nucleoprotein-specific CTLs.Abstract:
Cytotoxic T lymphocytes (CTLs) specific for conserved viral antigens can respond to different strains of virus, in contrast to antibodies, which are generally strain-specific. The generation of such CTLs in vivo usually requires endogenous expression of the antigen, as occurs in the case of virus infection. To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleoprotein was injected into the quadriceps of BALB/c mice. This resulted in the generation of nucleoprotein-specific CTLs and protection from a subsequent challenge with a heterologous strain of influenza A virus, as measured by decreased viral lung titers, inhibition of mass loss, and increased survival.read more
Citations
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DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations
Ellen F. Fynan,Robert G. Webster,Deborah H. Fuller,Joel R. Haynes,Joseph C. Santoro,Harriet L. Robinson +5 more
TL;DR: By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis, and 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 micrograms of DNA.
Journal ArticleDOI
DNA vaccines: immunology, application, and optimization*.
TL;DR: This review focuses on the mechanisms by which DNA vaccines elicit immune responses, and a list of potential applications in a variety of preclinical models is provided.
Patent
Induction of a protective immune response in a mammal by injecting a DNA sequence
TL;DR: In this paper, a method for delivering an isolated polynucleotide such as DNA or RNA to the interior of a cell in a mammal comprising the injection of an isolated POINTE into a muscle of the mammal where the poynucleotide is taken up by the cells of the muscle and exerts a therapeutic effect on the mammal.
Journal ArticleDOI
Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene Immunization
Yukio Sato,Mark Roman,H Tighe,Delphine J. Lee,Maripat Corr,Minh-Duc Nguyen,Gregg J. Silverman,Martin Lotz,Dennis A. Carson,Eyal Raz +9 more
TL;DR: Although ISS are necessary for gene vaccination, they down-regulate gene expression and thus may interfere with gene replacement therapy by inducing proinflammatory cytokines.
Journal ArticleDOI
DNA vaccines: ready for prime time?
TL;DR: A productive future for DNA vaccine technology is suggested as more optimized constructs, better trial designs and improved platforms are being brought into the clinic.
References
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Journal ArticleDOI
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