Journal ArticleDOI
Histone variants specific to the transcriptionally active, amitotically dividing macronucleus of the unicellular eucaryote, tetrahymena thermophila
TLDR
Primary sequence variants of the inner histones, presumably indicative of nucleosome heterogeneity, exist in a lower eucaryote, in an amitotic nucleus, and within the nucleus of a clonally propagated organism.About:
This article is published in Cell.The article was published on 1980-07-01. It has received 192 citations till now. The article focuses on the topics: Histone code & Macronucleus.read more
Citations
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Journal ArticleDOI
High-resolution profiling of histone methylations in the human genome.
Artem Barski,Suresh Cuddapah,Kairong Cui,Tae-Young Roh,Dustin E. Schones,Zhibin Wang,Gang Wei,Iouri Chepelev,Keji Zhao +8 more
TL;DR: High-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology are generated.
Journal ArticleDOI
Conserved Histone Variant H2A.Z Protects Euchromatin from the Ectopic Spread of Silent Heterochromatin
TL;DR: Euchromatin and heterochromatin each contains components that antagonize switching to the opposite chromatin state and Htz1 is enriched in these euchromatic regions and acts synergistically with a boundary element to prevent the spread of heterochromeatin.
Journal ArticleDOI
Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene.
TL;DR: In situ hybridization with these probes indicated that the gene maps to a single chromosome location at 29A on the 2L chromosome, which should be effective for cloning the genes and identifying the genetic loci of chromosomal proteins which cannot be readily assayed by other means.
Journal ArticleDOI
Repression domain of the yeast global repressor Tup1 interacts directly with histones H3 and H4.
TL;DR: It is reported that Tup1 directly interacts with the amino-terminal tails of histones H3 and H4, providing a molecular basis for this connection and suggesting a mechanism whereby the organization of chromatin may be modulated in response to changing environmental signals.
Journal ArticleDOI
Variant histone H2A.Z is globally localized to the promoters of inactive yeast genes and regulates nucleosome positioning.
Benoit Guillemette,Alain R. Bataille,Nicolas Gévry,Maryse Adam,Mathieu Blanchette,François Robert,Luc Gaudreau +6 more
TL;DR: The data suggest that the incorporation of H2A.Z into specific promoter-bound nucleosomes configures chromatin structure to poise genes for transcriptional activation, and the relevance of these findings to higher eukaryotes is discussed.
References
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Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
Journal ArticleDOI
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.
Journal ArticleDOI
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
TL;DR: It is demonstrated that pre-exposure of the film to a brief flash of light greatly increases the sensitivity of fluorography, because it corrects the non-linear relationship between radioactivity of the sample and absorbance of theFilm image.
Journal ArticleDOI
Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.
Ira L. Goldknopf,Harris Busch +1 more
TL;DR: It is indicated that protein A24 has two amino termini and one carboxyl terminus, and the branched structure ofprotein A24 proposed is: (see text).
Book ChapterDOI
Isolation of micro- and macronuclei of Tetrahymena pyriformis.
TL;DR: This chapter discusses the isolation of micro- and macronuclei of Tetrahymena Pyriformis and suggests that these two nuclei may be used as a model system to explore the mechanisms whereby the same genetic information is maintained in different structural and functional states in eukaryotic cells.