Hyphal induction in the human fungal pathogen Candida albicans reveals a characteristic wall protein profile
Clemens J. Heilmann,Alice G. Sorgo,Adriaan R. Siliakus,Henk L. Dekker,Stanley Brul,Chris G. de Koster,Leo J. de Koning,Frans M. Klis +7 more
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This is, to the authors' knowledge, the first systematic, quantitative analysis of the changes in the wall proteome of C. albicans upon hyphal induction and proposes new wall-protein-derived candidates for vaccine development.Abstract:
The ability of Candida albicans to switch from yeast to hyphal growth is essential for its virulence. The walls and especially the covalently attached wall proteins are involved in the primary host–pathogen interactions. Three hyphal induction methods were compared, based on fetal calf serum, the amino sugar N-acetylglucosamine (GlcNAc) and the mammalian cell culture medium Iscove’s modified Dulbecco’s medium (IMDM). GlcNAc and IMDM were preferred, allowing stable hyphal growth over a prolonged period without significant reversion to yeast growth and with high biomass yields. We employed Fourier transform-MS combined with a 15N-metabolically labelled reference culture as internal standard for relative quantification of changes in the wall proteome upon hyphal induction. A total of 21 wall proteins were quantified. Our induction methods triggered a similar response characterized by (i) a category of wall proteins showing strongly increased incorporation levels (Als3, Hwp2, Hyr1, Plb5 and Sod5), (ii) another category with strongly decreased levels (Rhd3, Sod4 and Ywp1) and (iii) a third one enriched for carbohydrate-active enzymes (including Cht2, Crh11, Mp65, Pga4, Phr1, Phr2 and Utr2) and showing only a limited response. This is, to our knowledge, the first systematic, quantitative analysis of the changes in the wall proteome of C. albicans upon hyphal induction. Finally, we propose new wall-protein-derived candidates for vaccine development.read more
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Book ChapterDOI
Fungal Cell Wall Organization and Biosynthesis
TL;DR: The composition and organization of the cell walls from Saccharomyces cerevisiae, Candida albicans, Aspergillus fumigatus, Schizosaccharomysts pombe, Neurospora crassa, and Cryptococcus neoformans are compared and contrasted as discussed by the authors.
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The Pathogen Candida albicans Hijacks Pyroptosis for Escape from Macrophages
Nathalie Uwamahoro,Jiyoti Verma-Gaur,Hsin-Hui Shen,Yue Qu,Rowena S. Lewis,Rowena S. Lewis,Jingxiong Lu,Keith R. Bambery,Seth L. Masters,Seth L. Masters,James E Vince,James E Vince,Thomas Naderer,Ana Traven +13 more
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Oxidative Stress Responses in the Human Fungal Pathogen, Candida albicans
Alessandra da Silva Dantas,Alison M. Day,Mélanie A. C. Ikeh,Iaroslava Kos,Beatrice Achan,Janet Quinn +5 more
TL;DR: This review presents an up-date of the current understanding of the role and regulation of oxidative stress responses in this important human fungal pathogen.
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Carbon source‐induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans
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TL;DR: It is shown that growth on the physiologically relevant carboxylic acid, lactate, has a significant impact on the C. albicans cell wall proteome and secretome and this plasticity influences important fitness and virulence attributes known to modulate the behavior of C.Albicans in different host microenvironments during infection.
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Antonio Cassone,Roberto Cauda +1 more
TL;DR: Overall, studies of Candida and candidiasis in the HIV-positive patient while certainly contributing to a more effective control of the microorganism may also provide useful information on HIV–host relationship itself that can assist the fight against the virus.
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