Imaging intracellular fluorescent proteins at nanometer resolution.
Eric Betzig,George H. Patterson,Rachid Sougrat,O. Wolf Lindwasser,Scott G. Olenych,Juan S. Bonifacino,Michael W. Davidson,Jennifer Lippincott-Schwartz,Harald F. Hess +8 more
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TLDR
This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.Abstract:
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.read more
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3D imaging of mammalian cells with ion-abrasion scanning electron microscopy.
TL;DR: IA-SEM is shown to be a useful tool for imaging large mammalian cells in their entirety at resolutions in the nanometer range and it is shown that 10nm-sized gold particles and quantum dot particles with 7 nm-sized cores can be detected in single cross-sectional images.
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Resolving sub-diffraction limit encounters in nanoparticle tracking using live cell plasmon coupling microscopy.
TL;DR: This work improves the resolving power of the optical microscope by detecting plasmon coupling between individual gold nanoparticle labels using a ratiometric detection scheme, and applies this to resolve the interparticle separations during individual encounters of gold nanoparticles labeled fibronectin-integrin complexes in living HeLa cells.
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Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy
TL;DR: In this paper, the Her2/neu tyrosine kinase receptor sites were labelled by immunofluorescence using conventional fluorescent dyes (Alexa conjugated antibodies).
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Planar Diffractive Lenses: Fundamentals, Functionalities, and Applications.
Kun Huang,Kun Huang,Fei Qin,Hong Liu,Huapeng Ye,Cheng-Wei Qiu,Minghui Hong,Boris Luk'yanchuk,Boris Luk'yanchuk,Boris Luk'yanchuk,Jinghua Teng +10 more
TL;DR: The recent advances in planar diffractive lenses (PDLs) are reviewed from a united theoretical account on diffraction-based focusing optics, and the underlying physics of nanofocusing via constructive or destructive interference is revealed.
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Speckle correlation resolution enhancement of wide-field fluorescence imaging
TL;DR: In this article, a high-index scattering medium was used as an imaging lens to enhance the resolution of two-dimensional fluorescence images of a collection of 100 nm diameter dye-doped nanospheres.
References
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TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
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Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
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Precise nanometer localization analysis for individual fluorescent probes
TL;DR: A localization algorithm motivated from least-squares fitting theory is constructed and tested both on image stacks of 30-nm fluorescent beads and on computer-generated images (Monte Carlo simulations), and results show good agreement with the derived precision equation.
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Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution
TL;DR: Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples, and a recently proposed method in which the nonlinearity arises from saturation of the excited state is experimentally demonstrated.
Journal ArticleDOI
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Ahmet Yildiz,Joseph N. Forkey,Sean A. McKinney,Taekjip Ha,Taekjip Ha,Yale E. Goldman,Paul R. Selvin,Paul R. Selvin +7 more
TL;DR: The results strongly support a hand-over-hand model of motility, not an inchworm model, which moves processively on actin.
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