Imaging intracellular fluorescent proteins at nanometer resolution.
Eric Betzig,George H. Patterson,Rachid Sougrat,O. Wolf Lindwasser,Scott G. Olenych,Juan S. Bonifacino,Michael W. Davidson,Jennifer Lippincott-Schwartz,Harald F. Hess +8 more
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TLDR
This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.Abstract:
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.read more
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Label-free photoacoustic nanoscopy
Amos Danielli,Konstantin Maslov,Alejandro Garcia-Uribe,Amy M. Winkler,Chiye Li,Lidai Wang,Yun Chen,Gerald W. Dorn,Lihong V. Wang +8 more
TL;DR: This work reports label-free photoacoustic (PA) nanoscopy, which is exquisitely sensitive to optical absorption, with an 88 nm resolution, which provides super-resolution imaging with optical sectioning.
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Graphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells
TL;DR: Graphene is utilized, a single-atom-thick carbon meshwork, as the thinnest possible impermeable and conductive membrane to protect animal cells from vacuum, thus enabling high-resolution electron microscopy of wet and untreated whole cells with exceptional ease.
Journal ArticleDOI
Modern fluorescent proteins: from chromophore formation to novel intracellular applications.
Olesya V. Stepanenko,Olga V. Stepanenko,Daria M. Shcherbakova,Irina M. Kuznetsova,Konstantin K. Turoverov,Vladislav V. Verkhusha +5 more
TL;DR: The current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FP, and fluorescent timers is reviewed.
Journal ArticleDOI
Rhodamine spiroamides for multicolor single-molecule switching fluorescent nanoscopy
Vladimir N. Belov,Mariano L. Bossi,Jonas Fölling,Vadim P. Boyarskiy,Vadim P. Boyarskiy,Stefan W. Hell +5 more
TL;DR: A series of rhodamine spiroamides is presented along with characterizations of their most relevant properties for application as fluorescent probes in single-molecule switching and localization microscopy, illustrating the potential of the labels in the colocalization of biological objects and the two-photon activation technique with optical sectioning.
Journal ArticleDOI
Roadmap on superoscillations
Michael V Berry,Nikolay I. Zheludev,Nikolay I. Zheludev,Yakir Aharonov,Fabrizio Colombo,Irene Sabadini,Daniele C. Struppa,Jeff Tollaksen,Edward T. F. Rogers,Fei Qin,Minghui Hong,Xiangang Luo,Roei Remez,Ady Arie,Jörg B. Götte,Jörg B. Götte,Mark R. Dennis,Alex M. H. Wong,George V. Eleftheriades,Yaniv Eliezer,Alon Bahabad,Gang Chen,Zhongquan Wen,Gaofeng Liang,Chenglong Hao,Cheng-Wei Qiu,Achim Kempf,Eytan Katzav,Moshe Schwartz +28 more
TL;DR: Super-oscillations are band-limited functions with the counterintuitive property that they can vary arbitrarily faster than their fastest Fourier component, over arbitrarily long intervals as discussed by the authors, which has implications for information theory and applications to optical vortices, sub-wavelength microscopy and related areas of nanoscience.
References
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TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
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Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Precise nanometer localization analysis for individual fluorescent probes
TL;DR: A localization algorithm motivated from least-squares fitting theory is constructed and tested both on image stacks of 30-nm fluorescent beads and on computer-generated images (Monte Carlo simulations), and results show good agreement with the derived precision equation.
Journal ArticleDOI
Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution
TL;DR: Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples, and a recently proposed method in which the nonlinearity arises from saturation of the excited state is experimentally demonstrated.
Journal ArticleDOI
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Ahmet Yildiz,Joseph N. Forkey,Sean A. McKinney,Taekjip Ha,Taekjip Ha,Yale E. Goldman,Paul R. Selvin,Paul R. Selvin +7 more
TL;DR: The results strongly support a hand-over-hand model of motility, not an inchworm model, which moves processively on actin.
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