Imaging intracellular fluorescent proteins at nanometer resolution.

doi:10.1126/SCIENCE.1127344

Open AccessJournal ArticleDOI

Imaging intracellular fluorescent proteins at nanometer resolution.

Eric Betzig, +8 more

- 15 Sep 2006 -

Science

- Vol. 313, Iss: 5793, pp 1642-1645

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TLDR

This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.

Abstract:

We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.

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