Imaging intracellular fluorescent proteins at nanometer resolution.
Eric Betzig,George H. Patterson,Rachid Sougrat,O. Wolf Lindwasser,Scott G. Olenych,Juan S. Bonifacino,Michael W. Davidson,Jennifer Lippincott-Schwartz,Harald F. Hess +8 more
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TLDR
This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.Abstract:
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to approximately 2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method--termed photoactivated localization microscopy--to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.read more
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The universal scaling laws that determine the achievable resolution in different schemes for super-resolution imaging
Philip R. Hemmer,Todd Zapata +1 more
TL;DR: In this article, the resolution limits of popular sub-diffraction and sub-wavelength imaging schemes are examined using a unified approach that allows rapid comparison of the relative merits and shortcomings of each technique.
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Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)
Stefan Geissbuehler,Noelia L. Bocchio,Claudio Dellagiacoma,Corinne Berclaz,Marcel Leutenegger,Theo Lasser +5 more
TL;DR: In this article, a balanced super-resolution optical fluctuation imaging (bSOFI) was proposed to cancel the nonlinear response to brightness and blinking heterogeneities in the sample, which limits the use of higher cumulant orders for improving the resolution.
Journal Article
Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination (vol 113, pg E5454, 2016)
Sophie V. Pageon,Thibault Tabarin,Yui Yamamoto,Yuanqing Ma,Philip R. Nicovich,John S. Bridgeman,Andre Cohnen,Carola Benzing,Yijun Gao,Michael D. Crowther,Katie Tungatt,Garry Dolton,Andrew K. Sewell,David Price,Oreste Acuto,Robert G. Parton,J. Justin Gooding,Jérémie Rossy,Jamie Rossjohn,Katharina Gaus +19 more
TL;DR: TCR–CD3 clustering translates antigen recognition by the TCR into signal initiation by the CD3 complex, and the formation of dense signaling-competent clusters is a process of antigen discrimination.
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A Mitochondrion-Specific Photoactivatable Fluorescence Turn-On AIE-Based Bioprobe for Localization Super-Resolution Microscope
Xinggui Gu,Engui Zhao,Teng Zhao,Miaomiao Kang,Miaomiao Kang,Chen Gui,Jacky Wing Yip Lam,Shengwang Du,Michael Ming-tak Loy,Ben Zhong Tang,Ben Zhong Tang +10 more
TL;DR: A novel mitochondrion-specific photo-activatable fluorescence turn-on bioprobe, named as o-TPE-ON+, is designed and readily prepared, operating through a new photoactivatable mechanism of photocyclodehydrogenation.
Journal ArticleDOI
Beating Rayleigh's Curse by Imaging Using Phase Information
TL;DR: A simple scheme is experimentally demonstrated that captures most of the information in the full electromagnetic field in the image plane and has a greatly improved ability to estimate the distance between a pair of closely separated sources, achieving near-quantum-limited performance and immunity to Rayleigh's curse.
References
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疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
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Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
TL;DR: Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope with strikingly increased clarity compared to both conventional and confocal microscopes.
Journal ArticleDOI
Precise nanometer localization analysis for individual fluorescent probes
TL;DR: A localization algorithm motivated from least-squares fitting theory is constructed and tested both on image stacks of 30-nm fluorescent beads and on computer-generated images (Monte Carlo simulations), and results show good agreement with the derived precision equation.
Journal ArticleDOI
Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution
TL;DR: Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples, and a recently proposed method in which the nonlinearity arises from saturation of the excited state is experimentally demonstrated.
Journal ArticleDOI
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Ahmet Yildiz,Joseph N. Forkey,Sean A. McKinney,Taekjip Ha,Taekjip Ha,Yale E. Goldman,Paul R. Selvin,Paul R. Selvin +7 more
TL;DR: The results strongly support a hand-over-hand model of motility, not an inchworm model, which moves processively on actin.
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