Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
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TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
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Journal ArticleDOI
Modifying enzyme activity and selectivity by immobilization.
Rafael C. Rodrigues,Claudia Ortiz,Ángel Berenguer-Murcia,Rodrigo Torres,Roberto Fernandez-Lafuente +4 more
TL;DR: In this tutorial review, some of the main reasons that may produce an improvement in enzyme activity, specificity or selectivity, either real or apparent, due to immobilization are listed.
Journal ArticleDOI
Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance
TL;DR: The advantages and disadvantages of the different existing immobilization strategies to solve the different aforementioned enzyme limitations are given and some advice to select the optimal strategy for each particular enzyme and process is given.
Journal ArticleDOI
Understanding enzyme immobilisation
TL;DR: This tutorial review focuses on the understanding of enzyme immobilisation, which can address the issue of enzymatic instability.
Journal ArticleDOI
Enzyme stability and stabilization—Aqueous and non-aqueous environment
TL;DR: An overview of the denaturation mechanisms in aqueous and non-aqueous environment is given in this article, and various methods of enzyme stabilization with respect to their use in the aqueously and nonaqueous environments have been given.
Journal ArticleDOI
Polymer biodegradation: mechanisms and estimation techniques.
Nathalie Lucas,Christophe Bienaimé,Christian Belloy,Michèle Queneudec,Françoise Silvestre,Jose-Edmundo Nava-Saucedo +5 more
TL;DR: The aim of this review is to emphasise the importance of measure as well as possible, the last stage of the biodegradation, in order to certify the integration of new materials into the biogeochemical cycles.
References
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Journal ArticleDOI
Preparation of a very stable immobilized biocatalyst of glucose oxidase from Aspergillus niger.
Lorena Betancor,Fernando López-Gallego,Aurelio Hidalgo,Noelia Alonso-Morales,Gisela Dellamora-Ortiz,Jose M. Guisan,Roberto Fernandez-Lafuente +6 more
TL;DR: The adsorption of GOX on aminated cationic support and subsequent treatment with glutaraldehyde was presented as a very successful methodology for achieving a very stable biocatalyst.
Journal ArticleDOI
Characterization of Thermoanaerobacter cyclomaltodextrin glucanotransferase immobilized on glyoxyl-agarose
TL;DR: The immobilization of the Thermoanaerobacter cyclomaltodextrin glucanotransferase (CGTase) enzyme into cross-linked 6% agarose beads activated by high density of linear aldehyde groups (glyoxyl-agarose) that allow the establishment of multi-attachment enzyme-support bonds are presented.
Journal ArticleDOI
Stabilization of heterodimeric enzyme by multipoint covalent immobilization: Penicillin G acylase from Kluyvera citrophila.
Jose M. Guisan,Gregorio Álvaro,Roberto Fernandez-Lafuente,Cristina M. Rosell,José Luis García,Auro Tagliani +5 more
TL;DR: In this article, the authors developed a strategy for immobilization-stabilization of penicillin G acylase (PGA) from Kluyvera citrophila by controlled multipoint covalent attachment to agarose-aldehyde gels.
Journal ArticleDOI
Biotransformations catalyzed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions.
Roberto Fernandez-Lafuente,Odette Hernández-Jústiz,Cesar Mateo,Marco Terreni,Gloria Fernández-Lorente,Miguel Moreno,Jorge Alonso,Jose L. Garcia-López,Jose M. Guisan +8 more
TL;DR: Synthetic yields improved from around 65%, under conditions where the nonstabilized derivative was stable, to around 85% in conditions where only the stabilized derivative could be utilized, and when using high concentrations of PGM, a significant worsening of the reaction performance was detected with a significant decrease in the yields.
Journal Article
Dissociative thermal inactivation, stability, and activity of oligomeric enzymes
TL;DR: The relations which allow the calculation from experimental data the following physicochemical constants which characterize the stability of oligomeric enzymes are given: the constant for the rate of irreversible change of monomeric protein, the equilibrium constant for dimer dissociation, and the rate constant for dissociation.