Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
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TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
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Different strategies to immobilize lipase from Geotrichum candidum: Kinetic and thermodynamic studies
Matheus M. Ferreira,Fernanda L.B. Santiago,Nádia A.G.da Silva,Jaine H.H. Luiz,Roberto Fernandez-Lafuente,Adriano A. Mendes,Daniela B. Hirata +6 more
TL;DR: The prepared biocatalyst prepared using purified GCLI immobilized on MANAE-agarose allowed a great increase in thermostability and was able to hydrolyze methyl phenylacetate better than methyl mandelate.
Journal ArticleDOI
A new bioprocess for the production of prebiotic lactosucrose by an immobilized β-galactosidase
Lovaine Silva Duarte,Jéssie da Natividade Schöffer,André Soibelmann Glock Lorenzoni,Rafael C. Rodrigues,Eliseu Rodrigues,Plinho Francisco Hertz +5 more
TL;DR: The results fulfill some important aspects for the enzyme immobilization and oligosaccharides synthesis: the simplicity of the protocols, the high operational stability of the enzyme and the possibility of driving the final products.
Journal ArticleDOI
Immobilization of horseradish peroxidase by electrospun fibrous membranes for adsorption and degradation of pentachlorophenol in water.
TL;DR: The operational and storage stability of immobilized HRP are highly improved through emulsion electrospinning, and the removal of PCP can be dramatically enhanced by the interaction of adsorbed PCP and HRP on the surface of EFMs.
Journal ArticleDOI
Site-Specific Protein Immobilization Using Unnatural Amino Acids
TL;DR: To develop this approach a range of solid-supports, organic linkers, and protein immobilization sites have been investigated using a GFP reporter system, and a resin-conferred stabilization was demonstrated in several organic solvents.
Journal ArticleDOI
Polyaniline-assisted silver nanoparticles: a novel support for the immobilization of α-amylase.
TL;DR: Polyaniline-assisted Ag nanocomposites were synthesized using ammonium peroxydisulfate as oxidant to support the covalent conjugation of α-amylase and retained significantly very high thermal stability.
References
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Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.