Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
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TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
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Biotechnological production of biodiesel fuel using biocatalysed transesterification: a review.
TL;DR: Optimisation research of lipase-catalysed transesterification could include development of new reactor systems with immobilised biocatalysts, the use of lipases tolerant to organic solvents, intracellular lipases and genetically modified microorganisms.
Journal ArticleDOI
Engineering Thermostable Microbial Xylanases Toward its Industrial Applications
TL;DR: The present review explores xylanase–substrate interactions using gene-editing tools toward the comprehension in improvement in industrial stability of xylanases.
Journal ArticleDOI
Lignocellulases: a review of emerging and developing enzymes, systems, and practices
Eugene M. Obeng,Siti Nurul Nadzirah Adam,Cahyo Budiman,Clarence M. Ongkudon,Ruth Maas,Joachim Jose +5 more
TL;DR: This review focuses on the lignocellulosic biocommodity pathway, with emphasis on cellulase and hemicellulase systems, and some of the prominent and influential discoveries in the cellulase fraternity have been discussed.
Journal ArticleDOI
Effect of immobilization rate and enzyme crowding on enzyme stability under different conditions. The case of lipase from Thermomyces lanuginosus immobilized on octyl agarose beads
Hadjer Zaak,El-Hocine Siar,Jakub F. Kornecki,Laura Fernandez-Lopez,Sara G. Pedrero,Jose J. Virgen-Ortíz,Roberto Fernandez-Lafuente +6 more
TL;DR: Results show that the TLL concentration during immobilization may greatly affect TLL properties, perhaps due to altering enzyme packing.
Journal ArticleDOI
Heterogeneous catalysts based on mesoporous metal–organic frameworks
TL;DR: Mesopores in MOFs can decline mass transfer and exchange resistance in a confined space, leading to the improvement of their catalytic performances as mentioned in this paper, but it is challenging to synthesize and apply meso-MOFs for catalysis owing to the requirement of a combination of good chemical stability and large pore size.
References
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Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.