Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
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TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
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Journal ArticleDOI
Selective Ethanolysis of Fish Oil Catalyzed by Immobilized Lipases
TL;DR: In this article, three lipases from Candida antarctica B (CALB), Thermomyces lanuginosa (TLL) and Rhizomucor miehei (RML) were studied.
Journal ArticleDOI
Characterization, Analysis, and Application of Fabricated Fe3O4-Chitosan-Pectinase Nanobiocatalyst
Muthulingam Seenuvasan,Kannaiyan Sathish Kumar,Carlin Geor Malar,Sridhar Preethi,Madhava Anil Kumar,Nagarajan Balaji +5 more
TL;DR: The investigation on fabrication of Fe3O4-chitosan-pectinase nanobiocatalyst was performed by covalently binding the pectinase onto carboxyl group activated chitOSan-coated magnetic nanoparticles (CMNPs) to monitor their improved activity, the pH, temperature, reusability, storage ability, and kinetic studies were established.
Book ChapterDOI
Biosynthesis, Purification and Biotechnological Use of Exopolysaccharides Produced by Lactic Acid Bacteria
María Laura Werning,Sara Notararigo,Montserrat Nácher,Pilar Fernández de Palencia,Rosa Aznar,Paloma López +5 more
TL;DR: This poster presents a probabilistic assessment of the phytochemical properties of the fruit extract of almonds and its applications in the context of animal nutrition and human health.
Journal ArticleDOI
Trypsin inhibitor screening in traditional Chinese medicine by using an immobilized enzyme microreactor in capillary and molecular docking study
TL;DR: The proposed method was successfully applied to screen trypsin inhibitors from 15 compounds of traditional Chinese medicine and it has been found that baicalin showed inhibitory potency.
Journal ArticleDOI
Immobilization–stabilization of an α-galactosidase from Thermus sp. strain T2 by covalent immobilization on highly activated supports: Selection of the optimal immobilization strategy
Miguel Filho,Benevides C. C. Pessela,Cesar Mateo,Alfonso V. Carrascosa,Roberto Fernandez-Lafuente,Jose M. Guisan +5 more
TL;DR: The best stability was obtained by immobilization on glutaraldehyde activated supports, which retained over 90% of initial activity and improved the optimal temperature from 65 °C (soluble enzyme) to 70 °C.
References
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Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.