Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
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TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
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Immobilization of xylanase on glutaraldehyde activated aluminum oxide pellets for increasing digestibility of poultry feed
TL;DR: In this paper, the authors reported covalent immobilization of xylanase by response surface methodology on glutaraldehyde-activated aluminum oxide pellets, which showed an increase in optimum temperature (from 50 to 60°C) and V max (from 3333.33 to 5000 IU/mL) in comparison with free enzyme.
Journal ArticleDOI
Enzyme production of D-gluconic acid and glucose oxidase: successful tales of cascade reactions
Jakub F. Kornecki,Diego Carballares,Paulo Waldir Tardioli,Rafael C. Rodrigues,Ángel Berenguer-Murcia,Andrés R. Alcántara,Roberto Fernandez-Lafuente +6 more
TL;DR: This review presents many different cascade reactions and discusses the advantages/drawbacks of the use of co-immobilized enzymes in the production of D-gluconic acid.
Journal ArticleDOI
Applications of enzymatic and non-enzymatic methods to access enantiomerically pure compounds using kinetic resolution and racemisation
TL;DR: Different methods to access enantiomerically pure compounds were discussed in this article, where a number of methods to obtain enantiomorphic pure compounds have been proposed, including the one described in this paper.
Journal ArticleDOI
Oxidation of phenolic compounds catalyzed by immobilized multi-enzyme systems with integrated hydrogen peroxide production
TL;DR: A novel multi-enzyme biocatalyst that has been immobilized on agarose-type carriers to oxidize phenols using oxygen and formic acid as indirect oxidants and can use a broad range of redox cofactors with similar oxidative effectiveness.
Journal ArticleDOI
Efficient Agaricus bisporus tyrosinase immobilization on cellulose-based carriers
TL;DR: A systematic study of the covalent immobilization of Agaricus bisporus tyrosinase on man-tailored cellulose-based carriers with different pore structure and functionalization showed that DEAE-Granocel with the largest mean pore diameter can offer highly active preparations with a monophenolase (L-Tyrosine) and diphenol enzyme activity immobilization efficiency of over 34% and 24%, respectively.
References
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Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.