Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
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TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
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Covalent attachment of trypsin on plasma polymerized allylamine.
Abdennour Abbas,Dominique Vercaigne-Marko,Philippe Supiot,B. Bocquet,Céline Vivien,Didier Guillochon +5 more
TL;DR: UV-vis absorption spectroscopy showed that allylamine plasma polymer presents a high adsorption yield of trypsin, and the highest catalytic activity obtained was for the enzymes that underwent the three steps of the immobilization process.
Journal ArticleDOI
Medium optimization and immobilization of purified fibrinolytic URAK from Bacillus cereus NK1 on PHB nanoparticles
Venkataraman Deepak,Shailaja Ilangovan,Madhumitha Vangal Sampathkumar,Maria Jacintha Victoria,Sheik Pran Babu Sardar Pasha,Sureshbabu Ram Kumar Pandian,Sangiliyandi Gurunathan +6 more
TL;DR: In this paper, an organism primarily isolated from soil, identified as Bacillus cereus NK1 was found to secrete an extracellular protease and the enzyme was identified to degrade fibrin.
Journal ArticleDOI
Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine
TL;DR: Polyethyleneimine modification on immobilized lipases greatly enhanced their enantioselectivity in the kinetic resolution of (±)-2-hydroxy-phenylacetic acid methyl ester and improved the stability of the biocatalyst at high temperatures and in the presence of high co-solvent concentrations.
Journal ArticleDOI
Superior activities of lipase immobilized on pure and hydrophobic clay supports: Characterization and catalytic activity studies
R. Reshmi,Sankaran Sugunan +1 more
TL;DR: Immobilized lipases exhibited enhanced storage stability than the native lipase (up to 40 days) and the free lipase was 195-fold more active in water compared to that in organic solvent.
Journal ArticleDOI
Enzyme activity assay for horseradish peroxidase encapsulated in peptide nanotubes.
TL;DR: It is proved that the encapsulation of HRP inside the PNT may be an effective way to keep the enzyme activity stable in various environments.
References
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Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.