Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
Reads0
Chats0
TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
More filters
Journal ArticleDOI
Enhancing Catalytic Performance of Porcine Pancreatic Lipase by Covalent Modification Using Functional Ionic Liquids
TL;DR: Various functional ionic liquids composed of different cations and anions were activated with carbonyldiimidazole and then covalently linked onto porcine pancreatic lipase (PPL) through lysin.
Journal ArticleDOI
Immobilized biocatalytic process development and potential application in membrane separation: a review
Sudip Chakraborty,Handajaya Rusli,Arijit Nath,Jaya Sikder,Chiranjib Bhattacharjee,Stefano Curcio,Enrico Drioli +6 more
TL;DR: The present research work highlights immobilization techniques, reactor setup, enzyme stability under immobilized conditions, the hydrodynamics of MBR, and its application, particularly, in the field of sugar, starch, drinks, milk, pharmaceutical industries and energy generation.
Journal ArticleDOI
Optimized preparation of CALB-CLEAs by response surface methodology: The necessity to employ a feeder to have an effective crosslinking
Jenniffer Cruz,Oveimar Barbosa,Rafael C. Rodrigues,Roberto Fernandez-Lafuente,Rodrigo Torres,Claudia Ortiz +5 more
TL;DR: In this article, the CLEA technique was used to immobilize the lipase B from Candida antarctica (CALB) using BSA-CLEA and bovine serum albumin was used as a feeder to improve the crosslinking.
Journal ArticleDOI
Alginate lyase immobilized chitosan nanoparticles of ciprofloxacin for the improved antimicrobial activity against the biofilm associated mucoid P. aeruginosa infection in cystic fibrosis
Krishna Kumar Patel,Muktanand Tripathi,Nidhi Pandey,Ashish Kumar Agrawal,Shilpkala Gade,Md. Meraj Anjum,Ragini Tilak,Sanjay Singh +7 more
TL;DR: Developed nanoparticles were found to have desired quality attributes and demonstrated sustained release following the Higuchi release kinetics and did not exhibit any toxicity in vitro MTT assay and in vivo on lungs male Wistar rats, suggesting the proposed strategy a better alternative for the effective treatment of cystic fibrosis infections.
Journal ArticleDOI
Candida antarctica Lipase B as Catalyst for Cyclic Esters Synthesis, Their Polymerization and Degradation of Aliphatic Polyesters
TL;DR: A review of the application of lipases in polymer chemistry covering the synthesis of cyclic ester monomers, linear and star oligomers, as well as well-defined high molar mass polymers with improved mechanical properties is presented in this article.
References
More filters
Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.