Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
Reads0
Chats0
TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
More filters
Journal ArticleDOI
Immobilization of pectinase on reusable polymer support for clarification of apple juice
TL;DR: Pectinase (E.C.15) was successfully immobilized on recyclable polymer matrix by reaction of polyethyleneimine (mol. wt. 70,000) with epoxy-activated acrylate copolymer DILBEAD-VWR as mentioned in this paper.
Journal ArticleDOI
Nanosponges: a potential nanocarrier for novel drug delivery-a review
TL;DR: Nanosponge technology has been explored for various applications like enhancing the bioavailability of drug molecules and delivery of drugs into the oral, topical as well as parenteral routes.
Journal ArticleDOI
Poly(N-vinylpyrrolidone)-poly(dimethylsiloxane)-based polymersome nanoreactors for laccase-catalyzed biotransformations.
TL;DR: Encapsulation of Lac in the polymersomes significantly protected the enzyme against enzymatic degradation and against small inhibitors: proteinase K caused 90% less degradation and the inhibitor sodium azide did not affect the enzyme's activity.
Journal ArticleDOI
Immobilization of chloroperoxidase onto highly hydrophilic polyethylene chains via bio-conjugation: Catalytic properties and stabilities
TL;DR: The increased thermal, storage and operational stability of immobilized CPO should depend on the creation of a comfortable strong hydrophilic microenvironment on the designed support to the host enzyme molecule.
Journal ArticleDOI
Nanozymes for regulation of reactive oxygen species and disease therapy
TL;DR: The catalytic mechanism of different activities of nanozyme-based platforms for ROS-related biological application is illustrated, and different strategies for using nanozymes to augment or reduce ROS level for the applications in cancer therapy, pathogen infection, neurodegeneration, etc.
References
More filters
Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.