Journal ArticleDOI
Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
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TLDR
In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.About:
This article is published in Enzyme and Microbial Technology.The article was published on 2007-05-02. It has received 3016 citations till now. The article focuses on the topics: Immobilized enzyme.read more
Citations
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A new heterofunctional amino-vinyl sulfone support to immobilize enzymes: Application to the stabilization of β-galactosidase from Aspergillus oryzae
TL;DR: The preparation of a new heterofunctional agarose support: amino-vinylsulfone has been employed to immobilize the interesting enzyme β-galactosidase from Aspergillus oryzae, and some covalent enzyme-support bonds could be formed, more rapidly at alkaline pH value.
Journal ArticleDOI
Perspectives and industrial potential of PGA selectivity and promiscuity
TL;DR: Biocatalytic applications exploiting enantioselectivity and promiscuity of prokaryotic PGAs that have been recently reported are discussed and presented with reaction substrates converted into active compounds useful for the pharmaceutical industry.
Journal ArticleDOI
Immobilization of pectinase on oxidized pulp fiber and its application in whitewater treatment.
TL;DR: A promising route to prepare available, cheap and biodegradable carrier for immobilizing enzymes with potential application in wastewater treatment in papermaking industry and when employed in whitewater treatment of paper making industry is demonstrated.
Journal ArticleDOI
Effective production of resistant starch using pullulanase immobilized onto magnetic chitosan/Fe3O4 nanoparticles.
TL;DR: Reusability results demonstrated that pullulanase immobilized by sol-gel encapsulation had the advantages of producing higher RS content as well as better operational stability compared to pullulAnase immobilization by cross-linking.
Journal ArticleDOI
Fabrication of a chitosan/glucose oxidase–poly(anilineboronic acid)–Aunano/Au-plated Au electrode for biosensor and biofuel cell
Yi Huang,Xiaoli Qin,Zou Li,Yingchun Fu,Cong Qin,Feng Wu,Zhaohong Su,Ming Ma,Qingji Xie,Shouzhuo Yao,Jiming Hu +10 more
TL;DR: The thus-prepared CS/GOx-PABA-Au(nano)/Au-plated Au electrode worked well in the first-/second generation biosensing modes and as a bioanode in a monopolar biofuel cell, with analytical or cell-power performance superior to those of most analogues hitherto reported.
References
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Journal ArticleDOI
Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.
TL;DR: An overview of glutaraldehyde as a crosslinking reagent is given by describing its structure and chemical properties in aqueous solution in an attempt to explain its high reactivity toward proteins, particularly as applied to the production of insoluble enzymes.
Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
TL;DR: The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Journal ArticleDOI
A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex
Andrzej M. Brzozowski,Andrzej M. Brzozowski,Urszula Derewenda,Urszula Derewenda,Zygmunt S. Derewenda,Zygmunt S. Derewenda,Guy Dodson,David M. R. Lawson,Johan P. Turkenburg,Fredrik Björkling,B. Huge-Jensen,S. A. Patkar,L. Thim +12 more
TL;DR: It is proposed that the structure of the enzyme in this complex of R. miehei lipase with n-hexylphosphonate ethyl ester is equivalent to the activated state generated by the oil–water interface.
Book
Enzymes in synthetic organic chemistry
TL;DR: Part 1 general aspects: rate acceleration in enzyme-catalyzed reactions Michaelis-Menten kinetics enzyme inhibition specificity improvement or alteration of enzyme specifity enzyme stabilization and reactor configuration cofactor regeneration enzyme catalysis in organic solvents multienzyme systems and metabolic engineering rational design of new enzymatic catalysts references.
Journal ArticleDOI
Enzymes for chemical synthesis
Kathryn M. Koeller,Chi-Huey Wong +1 more
TL;DR: Enzyme-catalysed chemical transformations are now widely recognized as practical alternatives to traditional (non-biological) organic synthesis, and as convenient solutions to certain intractable synthetic problems.