Journal ArticleDOI
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
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TLDR
The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase, linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin.Abstract:
In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively growing organs, under short- (6 h) and long- (2 d or 4 d) term and low (10 mM) and high (50 mM) doses of Cd, through a Methylation-Sensitive Amplification Polymorphism technique and an immunocytological approach, respectively. The expression of one member of the CHROMOMETHYLASE (CMT) family, a DNA methyltransferase, was also assessed by qRT-PCR. Nuclear chromatin ultrastructure was investigated by transmission electron microscopy. Cd treatment induced a DNA hypermethylation, as well as an up-regulation of CMT, indicating that de novo methylation did indeed occur. Moreover, a high dose of Cd led to a progressive heterochromatinization of interphase nuclei and apoptotic figures were also observed after long-term treatment. The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase. Such changes are linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin. Overall, the data show an epigenetic basis to the mechanism underlying Cd toxicity in plants.read more
Citations
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Journal ArticleDOI
MdNup54 Interactions With MdHSP70 Involved in Flowering in Apple
Chenguang Zhang,Xiaoshuang Zhang,Bo Cheng,Junkai Wu,Li-Bin Zhang,Xiaozhou Liao,Dong Zhang,Caiping Zhao,Na An,Mingyu Han,Libo Xing +10 more
TL;DR: Zhang et al. as mentioned in this paper analyzed the expression and function of MDNup54, a component of apple NPC, and found that the highest expression was found in the flower buds and maintained during 30-70 days after flowering.
Patent
Transgenic plants produced with a k-domain, and methods and expression cassettes related thereto
TL;DR: In this article, the K-domain of a MADS box gene was introduced to improve plant yield by introducing into a plant the K domain of the box gene, which provided plants with altered flower development, plant size and leaf development.
Journal ArticleDOI
Biomass yield in a genetically diverse Miscanthus sacchariflorus germplasm panel phenotyped at five locations in Asia, North America, and Europe
Joyce Njuguna,Lindsay V. Clark,Kossonou Guillaume Anzoua,Larisa Bagmet,Pavel Chebukin,Maria Stefanie Dwiyanti,Elena Dzyubenko,Nicolay Dzyubenko,Bimal Kumar Ghimire,Xiaoli Jin,Douglas H. Johnson,Uffe Jørgensen,Jens Bonderup Kjeldsen,Hironori Nagano,Junhua Peng,Karen Koefoed Petersen,Andrey Sabitov,Eun Soo Seong,Toshihiko Yamada,J. H. Yoo,Chang Yeon Yu,Hua Zhao,Stephen P. Long,Erik J. Sacks +23 more
TL;DR: In this article , a diversity panel of 605 M. sacchariflorus from six previously described genetic groups and 27 M. sinensis genotypes was phenotyped for dry biomass yield and 16 yield component traits, in field trials grown over 3 years at one subtropical location (Zhuji, China) and four temperate locations (Foulum, Denmark; Sapporo, Japan; Urbana, Illinois; and Chuncheon, South Korea).
Posted ContentDOI
Low-dose copper and blue light increases the yield of value-added biomolecules in Kirchneriella contorta (Chlorophyceae)
TL;DR: In this article , low-dose copper and blue/red lights were combined and used as a manipulative strategy to induce biomolecule accumulation in Kirchneriella contorta (Schmidle).
Book ChapterDOI
DNA methylation in plants and its role in abiotic stress tolerance
B. Divya Bhanu,B. Divya Bhanu,Anjani Alluri,Anjani Alluri,Arun K. Shanker,Kandasamy Ulaganathan +5 more
TL;DR: The role of DNA methylation as a stress tolerance mechanism is still in its infancy as discussed by the authors, however, it is a conserved change in the activity of the DNA, keeping the sequence unaltered.
References
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