Journal ArticleDOI
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
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The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase, linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin.Abstract:
In mammals, cadmium is widely considered as a non-genotoxic carcinogen acting through a methylation-dependent epigenetic mechanism. Here, the effects of Cd treatment on the DNA methylation patten are examined together with its effect on chromatin reconfiguration in Posidonia oceanica. DNA methylation level and pattern were analysed in actively growing organs, under short- (6 h) and long- (2 d or 4 d) term and low (10 mM) and high (50 mM) doses of Cd, through a Methylation-Sensitive Amplification Polymorphism technique and an immunocytological approach, respectively. The expression of one member of the CHROMOMETHYLASE (CMT) family, a DNA methyltransferase, was also assessed by qRT-PCR. Nuclear chromatin ultrastructure was investigated by transmission electron microscopy. Cd treatment induced a DNA hypermethylation, as well as an up-regulation of CMT, indicating that de novo methylation did indeed occur. Moreover, a high dose of Cd led to a progressive heterochromatinization of interphase nuclei and apoptotic figures were also observed after long-term treatment. The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase. Such changes are linked to nuclear chromatin reconfiguration likely to establish a new balance of expressed/repressed chromatin. Overall, the data show an epigenetic basis to the mechanism underlying Cd toxicity in plants.read more
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Early Events in Plant Abiotic Stress Signaling: Interplay Between Calcium, Reactive Oxygen Species and Phytohormones
Tapan Kumar Mohanta,Tufail Bashir,Abeer Hashem,Elsayed Fathi Abd_Allah,Abdul Latif Khan,Ahmed Al-Harrasi +5 more
TL;DR: Upon exposure to abiotic stresses, plants activate early stress-signaling mechanisms within a few seconds to a few hours to counter the stress responses and bring tolerance.
Journal ArticleDOI
Assessment for crop water stress with infrared thermal imagery in precision agriculture: A review and future prospects for deep learning applications
TL;DR: In this paper, the current practices of infrared thermal imagery utilized to assess crop water stress are reviewed along with the challenges and recommendations: (i) introduction of uncooled thermal camera and platforms, including ground-based platform and unmanned aerial vehicles (UAVs) platforms, for thermal imaging acquisition, (ii) strategies of canopy segmentation in thermal imaging used to obtain average canopy temperature for CWSI calculation, (iii) correlation between three forms of CWSI i.e., theoretical CWSI (CWSIt), empiricalCWSI (CWSIe), and statistic CW
Journal ArticleDOI
Metabolic profile and antioxidant responses during drought stress recovery in sugarcane treated with humic acids and endophytic diazotrophic bacteria
Natália Oliveira Aguiar,Leonardo Oliveira Medici,Fábio Lopes Olivares,Leonardo Barros Dobbss,A. Torres-Netto,Silézio Ferreira da Silva,Etelvino Henrique Novotny,L. P. Canellas +7 more
TL;DR: The PGPB-treated plants exhibited drought resistance that resembled ‘delayed stress onset’, which is a new term for preserving water in the plants tissues, resulting in plant water preservation.
Journal ArticleDOI
Characterization of a large sex determination region in Salix purpurea L. (Salicaceae)
Ran Zhou,David Macaya-Sanz,Eli Rodgers-Melnick,Craig H. Carlson,Fred E. Gouker,Luke M. Evans,Jeremy Schmutz,Jerry Jenkins,Juying Yan,Gerald A. Tuskan,Gerald A. Tuskan,Lawrence B. Smart,Stephen P. DiFazio +12 more
TL;DR: It is demonstrated that Salix purpurea also has a ZW system on chromosome 15, and raised the intriguing possibility of a translocation of the sex determination region within the Salicaceae lineage, suggesting a common evolutionary origin of the Populus and Salix sex determination loci.
Journal ArticleDOI
Pumpkin rootstock improves nitrogen use efficiency of watermelon scion by enhancing nutrient uptake, cytokinin content, and expression of nitrate reductase genes
Muhammad Azher Nawaz,Muhammad Azher Nawaz,Liangming Wang,Yanyan Jiao,Chen Chen,Liang Zhao,Mengjun Mei,Yunlong Yu,Zhilong Bie,Yuan Huang +9 more
TL;DR: PG improved the N use efficiency of watermelon scion through the vigorous root system of pumpkin rootstock that enhanced the uptake and accumulation of N, P, K, Ca, Mg, B, and Mn in watermelon.
References
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