In vivo two-photon microscopy to 1.6-mm depth in mouse cortex
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Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented, approximately reaching the fundamental depth limit in scattering tissue.Abstract:
Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.6 mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.read more
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In vivo three-photon microscopy of subcortical structures within an intact mouse brain
Nicholas G. Horton,Ke Wang,Demirhan Kobat,Catharine G. Clark,Frank W. Wise,Chris B. Schaffer,Chris Xu +6 more
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Through-skull fluorescence imaging of the brain in a new near-infrared window
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References
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The Mouse Brain in Stereotaxic Coordinates
TL;DR: The 3rd edition of this atlas is now in more practical 14"x11" format for convenient lab use and includes a CD of all plates and diagrams, as well as Adobe Illustrator files of the diagrams, and a variety of additional useful material.
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Deep tissue two-photon microscopy
Fritjof Helmchen,Winfried Denk +1 more
TL;DR: Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed.
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Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile Mice
TL;DR: A technique for two-photon fluorescence imaging with cellular resolution in awake, behaving mice with minimal motion artifact is reported, demonstrating that running-associated brain motion is limited to approximately 2-5 microm.
Journal ArticleDOI
Two-photon imaging to a depth of 1000 µm microm in living brains by use of a Ti:Al2O3 regenerative amplifier
TL;DR: It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses.
Journal ArticleDOI
Deep tissue multiphoton microscopy using longer wavelength excitation.
TL;DR: The maximal two-photon fluorescence microscopy (TPM) imaging depth achieved with 775-nm excitation is compared to that achieved with 1280- nm excitation through in vivo and ex vivo TPM of fluorescently-labeled blood vessels in mouse brain.