Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus
TLDR
A reverse-genetic system is described for the generation of recombinant coronaviruses based upon the in vitro transcription of infectious RNA from a cDNA copy of the human coronavirus 229E genome that has been cloned and propagated in vaccinia virus.Abstract:
The coronavirus genome is a positive-strand RNA of extraordinary size and complexity. It is composed of approximately 30000 nucleotides and it is the largest known autonomously replicating RNA. It is also remarkable in that more than two-thirds of the genome is devoted to encoding proteins involved in the replication and transcription of viral RNA. Here, a reverse-genetic system is described for the generation of recombinant coronaviruses. This system is based upon the in vitro transcription of infectious RNA from a cDNA copy of the human coronavirus 229E genome that has been cloned and propagated in vaccinia virus. This system is expected to provide new insights into the molecular biology and pathogenesis of coronaviruses and to serve as a paradigm for the genetic analysis of large RNA virus genomes. It also provides a starting point for the development of a new class of eukaryotic, multi-gene RNA vectors that are able to express several proteins simultaneously.read more
Citations
More filters
Book ChapterDOI
The molecular biology of coronaviruses.
TL;DR: This review summarizes both classical and contemporary discoveries in the study of the molecular biology of these infectious agents, with particular emphasis on the nature and recognition of viral receptors, viral RNA synthesis, and the molecular interactions governing virion assembly.
Journal ArticleDOI
Coronavirus biology and replication: implications for SARS-CoV-2.
TL;DR: The first discoveries that shape the current understanding of SARS-CoV-2 infection throughout the intracellular viral life cycle are summarized and relate that to the knowledge of coronavirus biology.
Journal ArticleDOI
Identification of a new human coronavirus
Lia van der Hoek,Krzysztof Pyrc,Maarten F. Jebbink,Wilma Vermeulen-Oost,Ron J. M. Berkhout,Katja C. Wolthers,Pauline M. E. Wertheim-van Dillen,Jos Kaandorp,Joke Spaargaren,Ben Berkhout +9 more
TL;DR: A new group 1 coronavirus, HCoV-NL63, was identified in a 7-month-old child suffering from bronchiolitis and conjunctivitis as discussed by the authors.
Journal ArticleDOI
Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus
TL;DR: The data gathered on the animal coronaviruses continue to be helpful in understanding SARS-CoV, and the progress in vaccine development and antiviral therapies is discussed.
Journal ArticleDOI
Mechanisms and enzymes involved in SARS coronavirus genome expression.
Volker Thiel,Konstantin A. Ivanov,Ákos Putics,Tobias Hertzig,Barbara Schelle,Sonja Bayer,Benedikt Weißbrich,Eric J. Snijder,Holger F. Rabenau,Hans Wilhelm Doerr,Alexander E. Gorbalenya,John Ziebuhr +11 more
TL;DR: The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.
References
More filters
Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Book ChapterDOI
The molecular biology of coronaviruses
Michael M. C. Lai,David Cavanagh +1 more
TL;DR: This chapter discusses the manipulation of clones of coronav virus and of complementary DNAs of defective-interfering RNAs to study coronavirus RNA replication, transcription, recombination, processing and transport of proteins, virion assembly, identification of cell receptors for coronaviruses, and processing of the polymerase.
Journal ArticleDOI
Virus-encoded proteinases and proteolytic processing in the Nidovirales.
TL;DR: This paper presents a meta-anatomy of the EMMARM, a probabilistic model of the immune response to EMT, which states that EMTs are more likely to attack the nervous system than the immune systems of mice.
Journal ArticleDOI
Retargeting of Coronavirus by Substitution of the Spike Glycoprotein Ectodomain: Crossing the Host Cell Species Barrier
TL;DR: FMHV is potentially the ideal recipient virus for carrying out reverse genetics of MHV by targeted RNA recombination, since it presents the possibility of selecting recombinants that have regained the ability to replicate in murine cells.
Related Papers (5)
Characterization of a novel coronavirus associated with severe acute respiratory syndrome.
Paul A. Rota,M. Steven Oberste,Stephan S. Monroe,W. Allan Nix,Ray Campagnoli,Joseph P. Icenogle,Silvia Peñaranda,Bettina Bankamp,Kaija Maher,Min hsin Chen,Suxiong Tong,Azaibi Tamin,Luis Lowe,Michael Frace,Joseph L. DeRisi,Qi Chen,David Wang,Dean D. Erdman,Teresa C. T. Peret,Cara C. Burns,Thomas G. Ksiazek,Pierre E. Rollin,Anthony Sanchez,Stephanie L. Liffick,Brian P. Holloway,Josef Limor,Karen A. McCaustland,Mellissa Olsen-Rasmussen,Ron A. M. Fouchier,Stephan Günther,Albert Osterhaus,Christian Drosten,Mark A. Pallansch,Larry J. Anderson,William J. Bellini +34 more
Identification of a novel coronavirus in patients with severe acute respiratory syndrome.
Christian Drosten,Stephan Günther,Wolfgang Preiser,Sylvie van der Werf,H. R. Brodt,Stephan Becker,Holger F. Rabenau,Marcus Panning,Larissa Kolesnikova,Ron A. M. Fouchier,Annemarie Berger,Ana-Maria Burguière,Jindrich Cinatl,Markus Eickmann,Nicolas Escriou,Klaus Grywna,Stefanie Kramme,Jean-Claude Manuguerra,Stefanie Müller,Volker Rickerts,Martin Stürmer,Simon Vieth,Hans-Dieter Klenk,Albert D. M. E. Osterhaus,Herbert Schmitz,Hans Wilhelm Doerr +25 more