Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Johan Ulrik Lind,Johan Ulrik Lind,Travis Alexander Busbee,Travis Alexander Busbee,Alexander D. Valentine,Alexander D. Valentine,Francesco S. Pasqualini,Francesco S. Pasqualini,Hongyan Yuan,Hongyan Yuan,Hongyan Yuan,Moran Yadid,Moran Yadid,Sung-Jin Park,Sung-Jin Park,Arda Kotikian,Arda Kotikian,Alexander P. Nesmith,Alexander P. Nesmith,Patrick H. Campbell,Patrick H. Campbell,Joost J. Vlassak,Jennifer A. Lewis,Jennifer A. Lewis,Kevin Kit Parker,Kevin Kit Parker +25 more
Reads0
Chats0
TLDR
Six functional inks are designed, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues via multi-material 3D printing.Abstract:
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multimaterial three-dimensional (3D) printing. Specifically, we designed six functional inks, based on piezo-resistive, high-conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readouts of tissue contractile stresses inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell-derived laminar cardiac tissues over four weeks.read more
Citations
More filters
Book ChapterDOI
3D bioprinting: An innovative technique for biofabrication applied to regenerative medicine and tissue engineering
TL;DR: In this paper , the potential of polymers and cells for their applications in 3D bioprinting of different biomaterials and printed scaffolds to form various tissues exploring their potential applications in tissue engineering and regenerative medicines.
Posted ContentDOI
Comparative study of evolutionary machine learning approaches to simulate the rheological characteristics of polybutylene succinate (PBS) utilized for fused deposition modeling (FDM)
TL;DR: In this paper , four types of machine learning (ML) approaches were used to predict the rheological degradation of polybutylene succinate, including artificial neural network, support vector regression, polynomial chaos expansion (PCE), and response surface model.
Journal ArticleDOI
Sensors-integrated organ-on-a-chip for biomedical applications
TL;DR: In this article , the authors discuss the recent research advances made with respect to sensors-integrated organ-on-a-chip in this overall review, and highlight the highlighted applications of different types of organ on a-chip incorporated with various sensors.
References
More filters
Journal ArticleDOI
Skin-like pressure and strain sensors based on transparent elastic films of carbon nanotubes
Darren J. Lipomi,Michael Vosgueritchian,Benjamin C. K. Tee,Sondra L. Hellstrom,Jennifer A. Lee,Courtney H. Fox,Zhenan Bao +6 more
TL;DR: Transparent, conducting spray-deposited films of single-walled carbon nanotubes are reported that can be rendered stretchable by applying strain along each axis, and then releasing this strain.
Journal ArticleDOI
Microfluidic organs-on-chips
TL;DR: A microfluidic cell culture device created with microchip manufacturing methods that contains continuously perfused chambers inhabited by living cells arranged to simulate tissue- and organ-level physiology has great potential to advance the study of tissue development, organ physiology and disease etiology.
Journal ArticleDOI
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Jordan S. Miller,Kelly R. Stevens,Michael T. Yang,Brendon M. Baker,Duc-Huy T. Nguyen,Daniel M. Cohen,Esteban Toro,Alice A. Chen,Peter A. Galie,Xiang-Qing Yu,Ritika Chaturvedi,Sangeeta N. Bhatia,Sangeeta N. Bhatia,Christopher S. Chen +13 more
TL;DR: 3D printed rigid filament networks of carbohydrate glass are used as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow.
Journal ArticleDOI
Direct ink writing of 3D functional materials
TL;DR: The ability to pattern materials in 3D shapes without the need for expensive tooling, dies, or lithographic masks is critical for composites, microfluidics, photonics, and tissue engineering as discussed by the authors.
Journal ArticleDOI
Three-dimensional bioprinting of thick vascularized tissues.
TL;DR: A multimaterial 3D bioprinting method is reported that enables the creation of thick human tissues (>1 cm) replete with an engineered extracellular matrix, embedded vasculature, and multiple cell types that can be actively perfused for long durations.