Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Johan Ulrik Lind,Johan Ulrik Lind,Travis Alexander Busbee,Travis Alexander Busbee,Alexander D. Valentine,Alexander D. Valentine,Francesco S. Pasqualini,Francesco S. Pasqualini,Hongyan Yuan,Hongyan Yuan,Hongyan Yuan,Moran Yadid,Moran Yadid,Sung-Jin Park,Sung-Jin Park,Arda Kotikian,Arda Kotikian,Alexander P. Nesmith,Alexander P. Nesmith,Patrick H. Campbell,Patrick H. Campbell,Joost J. Vlassak,Jennifer A. Lewis,Jennifer A. Lewis,Kevin Kit Parker,Kevin Kit Parker +25 more
Reads0
Chats0
TLDR
Six functional inks are designed, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues via multi-material 3D printing.Abstract:
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multimaterial three-dimensional (3D) printing. Specifically, we designed six functional inks, based on piezo-resistive, high-conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readouts of tissue contractile stresses inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell-derived laminar cardiac tissues over four weeks.read more
Citations
More filters
Journal ArticleDOI
Tissue-engineered heart chambers as a platform technology for drug discovery and disease modeling.
TL;DR: A review of tissue-engineered heart chambers can be found in this article , where the authors discuss challenges and prospects of tissue engineering for drug screening and disease modeling applications, as well as 3D bioprinting of functional cardiac tissue.
Journal ArticleDOI
Simulation of hypoxia of myocardial cells in microfluidic systems
TL;DR: A newly designed microfluidic system that allows simulation of myocardial hypoxia by biochemical method could be used to develop new methods of treatment of ischemic heart disease for example in cell therapy based on stem cells.
Journal ArticleDOI
Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance.
Andrew J. Capel,Matthew A. A. Smith,Silvia Taccola,Maria Pardo-Figuerez,Rowan P. Rimington,Mark P. Lewis,Steven D. R. Christie,Robert W. Kay,Russell A. Harris +8 more
TL;DR: In this paper, the ability of aerosol jet printing (AJP) to print digitally controlled patterns that influence neuronal guidance has been demonstrated, which consist of patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks.
Journal ArticleDOI
A path-following simulation-based study of elastic instabilities in nearly-incompressible confined cylinders under tension
TL;DR: In this paper, a robust numerical framework addressing the challenges that emerge in the simulation of this complex material response from the onset of instability to the post-bifurcation behavior is presented, which can be overcome by using sufficiently high order of interpolation in the finite element approximation, an arc-length-based nonlinear solution procedure that follows the entire equilibrium path of the system, and an implementation enabling parallel, large-scale simulations.
Journal ArticleDOI
A novel Roll Porous Scaffold 3D bioprinting technology
TL;DR: The ways to overcome the main technological barriers and to accelerate biofabrication of 3D cellular tissue constructs greatly, to build multi-cell implantations of high density and accuracy for vascular system are described.
References
More filters
Journal ArticleDOI
Skin-like pressure and strain sensors based on transparent elastic films of carbon nanotubes
Darren J. Lipomi,Michael Vosgueritchian,Benjamin C. K. Tee,Sondra L. Hellstrom,Jennifer A. Lee,Courtney H. Fox,Zhenan Bao +6 more
TL;DR: Transparent, conducting spray-deposited films of single-walled carbon nanotubes are reported that can be rendered stretchable by applying strain along each axis, and then releasing this strain.
Journal ArticleDOI
Microfluidic organs-on-chips
TL;DR: A microfluidic cell culture device created with microchip manufacturing methods that contains continuously perfused chambers inhabited by living cells arranged to simulate tissue- and organ-level physiology has great potential to advance the study of tissue development, organ physiology and disease etiology.
Journal ArticleDOI
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Jordan S. Miller,Kelly R. Stevens,Michael T. Yang,Brendon M. Baker,Duc-Huy T. Nguyen,Daniel M. Cohen,Esteban Toro,Alice A. Chen,Peter A. Galie,Xiang-Qing Yu,Ritika Chaturvedi,Sangeeta N. Bhatia,Sangeeta N. Bhatia,Christopher S. Chen +13 more
TL;DR: 3D printed rigid filament networks of carbohydrate glass are used as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow.
Journal ArticleDOI
Direct ink writing of 3D functional materials
TL;DR: The ability to pattern materials in 3D shapes without the need for expensive tooling, dies, or lithographic masks is critical for composites, microfluidics, photonics, and tissue engineering as discussed by the authors.
Journal ArticleDOI
Three-dimensional bioprinting of thick vascularized tissues.
TL;DR: A multimaterial 3D bioprinting method is reported that enables the creation of thick human tissues (>1 cm) replete with an engineered extracellular matrix, embedded vasculature, and multiple cell types that can be actively perfused for long durations.