Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Johan Ulrik Lind,Johan Ulrik Lind,Travis Alexander Busbee,Travis Alexander Busbee,Alexander D. Valentine,Alexander D. Valentine,Francesco S. Pasqualini,Francesco S. Pasqualini,Hongyan Yuan,Hongyan Yuan,Hongyan Yuan,Moran Yadid,Moran Yadid,Sung-Jin Park,Sung-Jin Park,Arda Kotikian,Arda Kotikian,Alexander P. Nesmith,Alexander P. Nesmith,Patrick H. Campbell,Patrick H. Campbell,Joost J. Vlassak,Jennifer A. Lewis,Jennifer A. Lewis,Kevin Kit Parker,Kevin Kit Parker +25 more
TLDR
Six functional inks are designed, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues via multi-material 3D printing.Abstract:
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multimaterial three-dimensional (3D) printing. Specifically, we designed six functional inks, based on piezo-resistive, high-conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readouts of tissue contractile stresses inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell-derived laminar cardiac tissues over four weeks.read more
Citations
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Instrumented Microphysiological Systems for Real-Time Measurement and Manipulation of Cellular Electrochemical Processes.
TL;DR: An overview of the sensing techniques that are relevant to MPS development are provided and the different organ systems to integrate instrumentation for measurement and manipulation of cellular function are highlighted.
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Multifunctional 3D electrode platform for real-time in situ monitoring and stimulation of cardiac tissues
Ning Zhang,Flurin Stauffer,Benjamin R. Simona,Feng Zhang,Zhao-Ming Zhang,Ning-Ping Huang,Janos Vörös +6 more
TL;DR: A platinum based 3D pillar electrode platform with cell growth guiding channel, which allows integrated, continuous electrical stimulation and recording of the cardiac tissues and has a potential to be applied in drug screening for in situ monitoring the biophysical parameters of the heart tissue in real-time.
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TL;DR: In this paper, the authors review the recent progress in 3D bioprinting for cardiac tissue engineering (CTE) and discuss several crucial challenges and present their perspective on 3D bio-printing techniques in the field of CTE.
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Modification of 3D printed PCL scaffolds by PVAc and HA to enhance cytocompatibility and osteogenesis
TL;DR: The 3D printed scaffold based on PCL/PVAc/HA tri-component system is a promising prospect for future individualized bone repair applications.
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3D Printing-Enabled Nanoparticle Alignment: A Review of Mechanisms and Applications
Weiheng Xu,Sayli Jambhulkar,Dharneedar Ravichandran,Yuxiang Zhu,Mounika Kakarla,Qiong Nian,Bruno Azeredo,Xiangfan Chen,Kailong Jin,Brent Vernon,David G. Lott,Jeffrey L. Cornella,Orit Shefi,Guillaume Miquelard-Garnier,Yang Yang,Kenan Song +15 more
TL;DR: In this paper, a review of the 3D printing-enabled nanoparticle alignment in well-established and in-house customized 3D-printing mechanisms that can lead to selective deposition and preferential orientation of nanoparticles is presented.
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