Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Johan Ulrik Lind,Johan Ulrik Lind,Travis Alexander Busbee,Travis Alexander Busbee,Alexander D. Valentine,Alexander D. Valentine,Francesco S. Pasqualini,Francesco S. Pasqualini,Hongyan Yuan,Hongyan Yuan,Hongyan Yuan,Moran Yadid,Moran Yadid,Sung-Jin Park,Sung-Jin Park,Arda Kotikian,Arda Kotikian,Alexander P. Nesmith,Alexander P. Nesmith,Patrick H. Campbell,Patrick H. Campbell,Joost J. Vlassak,Jennifer A. Lewis,Jennifer A. Lewis,Kevin Kit Parker,Kevin Kit Parker +25 more
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TLDR
Six functional inks are designed, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues via multi-material 3D printing.Abstract:
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multimaterial three-dimensional (3D) printing. Specifically, we designed six functional inks, based on piezo-resistive, high-conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readouts of tissue contractile stresses inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell-derived laminar cardiac tissues over four weeks.read more
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The bioprinting roadmap
Wei Sun,Wei Sun,Binil Starly,Andrew C. Daly,Jason A. Burdick,Juergen Groll,Gregor Skeldon,Gregor Skeldon,Wenmiao Shu,Yasuyuki Sakai,Marie Shinohara,Masaki Nishikawa,Jinah Jang,Dong-Woo Cho,Minghao Nie,Shoji Takeuchi,Serge Ostrovidov,Ali Khademhosseini,Roger D. Kamm,Vladimir Mironov,Lorenzo Moroni,Ibrahim T. Ozbolat +21 more
TL;DR: This bioprinting roadmap features salient advances in selected applications of the technique and highlights the status of current developments and challenges, as well as envisioned advances in science and technology, to address the challenges to the young and evolving technique.
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3D Bioprinting: from Benches to Translational Applications.
Marcel A. Heinrich,Marcel A. Heinrich,Wanjun Liu,Wanjun Liu,Andrea Jimenez,Jingzhou Yang,Ali Akpek,Ali Akpek,Ali Akpek,Xiao Liu,Xiao Liu,Qingmeng Pi,Qingmeng Pi,Xuan Mu,Ning Hu,Ning Hu,Raymond M. Schiffelers,Jai Prakash,Jingwei Xie,Yu Shrike Zhang +19 more
TL;DR: The history of bioprinting and the most recent advances in instrumentation and methods are covered, and the requirements for bioinks and cells to achieve optimal fabrication of biomimetic constructs are focused on.
Journal ArticleDOI
A New 3D Printing Strategy by Harnessing Deformation, Instability, and Fracture of Viscoelastic Inks
Hyunwoo Yuk,Xuanhe Zhao +1 more
TL;DR: A new strategy to exceed the limits of DIW 3D printing by harnessing deformation, instability, and fracture of viscoelastic inks is reported and a single nozzle can print fibers with resolution much finer than the nozzle diameter by stretching the extruded ink.
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Organ-On-A-Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies.
Samad Ahadian,Robert A. Civitarese,Dawn Bannerman,Mohammad Hossein Mohammadi,Rick Xing Ze Lu,Erika Yan Wang,Locke Davenport-Huyer,Ben Lai,Boyang Zhang,Yimu Zhao,Serena Mandla,Anastasia Korolj,Milica Radisic +12 more
TL;DR: The latest developments in OOC platforms (e.g., liver, skeletal muscle, cardiac, cancer, lung, skin, bone, and brain) are discussed as functional tools in simulating human physiology and metabolism.
Journal ArticleDOI
3D bioprinted functional and contractile cardiac tissue constructs.
TL;DR: The feasibility of 3D bioprinting functional cardiac tissues that could be used for tissue engineering applications and pharmaceutical purposes is demonstrated, and Notch signaling blockade significantly accelerated development and maturation of biopprinted cardiac tissues.
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