Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Johan Ulrik Lind,Johan Ulrik Lind,Travis Alexander Busbee,Travis Alexander Busbee,Alexander D. Valentine,Alexander D. Valentine,Francesco S. Pasqualini,Francesco S. Pasqualini,Hongyan Yuan,Hongyan Yuan,Hongyan Yuan,Moran Yadid,Moran Yadid,Sung-Jin Park,Sung-Jin Park,Arda Kotikian,Arda Kotikian,Alexander P. Nesmith,Alexander P. Nesmith,Patrick H. Campbell,Patrick H. Campbell,Joost J. Vlassak,Jennifer A. Lewis,Jennifer A. Lewis,Kevin Kit Parker,Kevin Kit Parker +25 more
Reads0
Chats0
TLDR
Six functional inks are designed, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues via multi-material 3D printing.Abstract:
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multimaterial three-dimensional (3D) printing. Specifically, we designed six functional inks, based on piezo-resistive, high-conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readouts of tissue contractile stresses inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell-derived laminar cardiac tissues over four weeks.read more
Citations
More filters
Journal ArticleDOI
3D printing approaches for cardiac tissue engineering and role of immune modulation in tissue regeneration.
TL;DR: An overview of 3D printed approaches for cardiac tissue engineering, and advances in 3D bioprinting of cardiac construct and models are presented and role of immune modulation to promote tissue regeneration is discussed.
Journal ArticleDOI
3D-Printed Biosensor Arrays for Medical Diagnostics
TL;DR: 3D printing in the fabrication of microfluidics, supporting equipment, and optical and electronic components of diagnostic devices is presented and emerging diagnostics systems using 3D bioprinting as a tool to incorporate living cells or biomaterials into 3D printing is reviewed.
Journal ArticleDOI
Toward the development of biomimetic injectable and macroporous biohydrogels for regenerative medicine.
Killian Flegeau,Richard Pace,Hélène Gautier,Gildas Rethore,Jérôme Guicheux,Jérôme Guicheux,Catherine Le Visage,Pierre Weiss +7 more
TL;DR: A paradigm shift is occurring where the combined efforts of fundamental and applied sciences head toward the development of hydrogels restoring tissue functions, serving as drug screening platforms or recreating complex organs.
Journal ArticleDOI
Advanced Material Strategies for Next-Generation Additive Manufacturing
Jinke Chang,Jiankang He,Mao Mao,Wenxing Zhou,Qi Lei,Xiao Li,Dichen Li,Chee Kai Chua,Xin Zhao +8 more
TL;DR: A state-of-the-art review on advanced material strategies for novel additive manufacturing processes is provided, mainly including conductive materials, biomaterials, and smart materials, which will significantly widen the application fields of next-generation additive manufacturing.
Journal ArticleDOI
Bioprinting of 3D tissues/organs combined with microfluidics
Jingyun Ma,Yachen Wang,Jing Liu +2 more
TL;DR: Advances in microfluidics-assisted bioprinting in the engineering of tissues/organs are discussed and future perspectives for this combination in the generation of highly biomimetic tissues and organs in vitro are provided.
References
More filters
Journal ArticleDOI
Skin-like pressure and strain sensors based on transparent elastic films of carbon nanotubes
Darren J. Lipomi,Michael Vosgueritchian,Benjamin C. K. Tee,Sondra L. Hellstrom,Jennifer A. Lee,Courtney H. Fox,Zhenan Bao +6 more
TL;DR: Transparent, conducting spray-deposited films of single-walled carbon nanotubes are reported that can be rendered stretchable by applying strain along each axis, and then releasing this strain.
Journal ArticleDOI
Microfluidic organs-on-chips
TL;DR: A microfluidic cell culture device created with microchip manufacturing methods that contains continuously perfused chambers inhabited by living cells arranged to simulate tissue- and organ-level physiology has great potential to advance the study of tissue development, organ physiology and disease etiology.
Journal ArticleDOI
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Jordan S. Miller,Kelly R. Stevens,Michael T. Yang,Brendon M. Baker,Duc-Huy T. Nguyen,Daniel M. Cohen,Esteban Toro,Alice A. Chen,Peter A. Galie,Xiang-Qing Yu,Ritika Chaturvedi,Sangeeta N. Bhatia,Sangeeta N. Bhatia,Christopher S. Chen +13 more
TL;DR: 3D printed rigid filament networks of carbohydrate glass are used as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow.
Journal ArticleDOI
Direct ink writing of 3D functional materials
TL;DR: The ability to pattern materials in 3D shapes without the need for expensive tooling, dies, or lithographic masks is critical for composites, microfluidics, photonics, and tissue engineering as discussed by the authors.
Journal ArticleDOI
Three-dimensional bioprinting of thick vascularized tissues.
TL;DR: A multimaterial 3D bioprinting method is reported that enables the creation of thick human tissues (>1 cm) replete with an engineered extracellular matrix, embedded vasculature, and multiple cell types that can be actively perfused for long durations.