Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Johan Ulrik Lind,Johan Ulrik Lind,Travis Alexander Busbee,Travis Alexander Busbee,Alexander D. Valentine,Alexander D. Valentine,Francesco S. Pasqualini,Francesco S. Pasqualini,Hongyan Yuan,Hongyan Yuan,Hongyan Yuan,Moran Yadid,Moran Yadid,Sung-Jin Park,Sung-Jin Park,Arda Kotikian,Arda Kotikian,Alexander P. Nesmith,Alexander P. Nesmith,Patrick H. Campbell,Patrick H. Campbell,Joost J. Vlassak,Jennifer A. Lewis,Jennifer A. Lewis,Kevin Kit Parker,Kevin Kit Parker +25 more
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TLDR
Six functional inks are designed, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues via multi-material 3D printing.Abstract:
Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native tissues in vitro, have emerged as a promising alternative. However, current MPS typically lack integrated sensors and their fabrication requires multi-step lithographic processes. Here, we introduce a facile route for fabricating a new class of instrumented cardiac microphysiological devices via multimaterial three-dimensional (3D) printing. Specifically, we designed six functional inks, based on piezo-resistive, high-conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues. We validated that these embedded sensors provide non-invasive, electronic readouts of tissue contractile stresses inside cell incubator environments. We further applied these devices to study drug responses, as well as the contractile development of human stem cell-derived laminar cardiac tissues over four weeks.read more
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Improved Resolution and Fidelity of Droplet-Based Bioprinting by Upward Ejection.
Yuan Ji,Qingzhen Yang,Guoyou Huang,Mingguang Shen,Zhen Jian,Marie-Jean Thoraval,Qin Lian,Xiaohui Zhang,Feng Xu +8 more
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Organs-on-a-chip: A new paradigm for toxicological assessment and preclinical drug development
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Additive manufacturing of three-dimensional (3D) microfluidic-based microelectromechanical systems (MEMS) for acoustofluidic applications
Ellen Cesewski,Alexander P. Haring,Yuxin Tong,Manjot Singh,Rajan Thakur,Sahil Laheri,Kaitlin A. Read,Michael D. Powell,Kenneth J. Oestreich,Kenneth J. Oestreich,Blake N. Johnson +10 more
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Design and fabrication of an integrated heart-on-a-chip platform for construction of cardiac tissue from human iPSC-derived cardiomyocytes and in situ evaluation of physiological function.
Feng Zhang,Kai-Yun Qu,Bin Zhou,Yong Luo,Zhen Zhu,Dejing Pan,Chang Cui,Yue Zhu,Ming-Long Chen,Ning-Ping Huang +9 more
TL;DR: In this article, a heart-on-a-chip device that contains microfluidic channels for long-term dynamic culture of cells, platinum wire electrodes for electrical stimulation of human induced pluripotent stem cells (hiPSC-CMs), and gold electrode arrays as acquisition electrodes for real-time recording electrophysiological signals of cardiac tissues was developed.
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