PatentDOI
Interferometric scattering microscopy
Philipp Kukura,Alexander Weigel +1 more
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TLDR
In this article, an interferometric scattering microscope is adapted by performing spatial filtering of output light, which comprises both light scattered from a sample location and illuminating light reflected from the sample location, prior to detection of the output light.Abstract:
An interferometric scattering microscope is adapted by performing spatial filtering of output light, which comprises both light scattered from a sample location and illuminating light reflected from the sample location, prior to detection of the output light. The spatial filtering passes the reflected illumination light but with a reduction in intensity that is greater within a predetermined numerical aperture than at larger numerical apertures. This enhances the imaging contrast for coherent illumination, particularly for objects that are weak scatterers.read more
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Adaptive dynamic range shift (ADRIFT) quantitative phase imaging.
TL;DR: In this article, the authors proposed and demonstrated supersensitive QPI with an expanded dynamic range enabled by adaptive dynamic range shift through a combination of wavefront shaping and dark-field QPI techniques.
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Quantifying the heterogeneity of macromolecular machines by mass photometry.
Adar Sonn-Segev,Katarina Belacic,Tatyana Bodrug,Gavin Young,Ryan T. VanderLinden,Brenda A. Schulman,Brenda A. Schulman,Johannes Schimpf,Thorsten Friedrich,Phat Vinh Dip,Thomas U. Schwartz,Benedikt Bauer,Jan-Michael Peters,Weston B. Struwe,Justin L. P. Benesch,Nicholas G. Brown,David Haselbach,Philipp Kukura +17 more
TL;DR: It is shown that mass photometry can accurately characterize the heterogeneity of a sample using minimal material with high resolution within a matter of minutes, with implications for structural studies specifically and in vitro studies more generally.
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Operando optical tracking of single-particle ion dynamics in batteries.
TL;DR: A simple laboratory-based, optical interferometric scattering microscope is introduced to resolve nanoscopic lithium-ion dynamics in battery materials, and is applied to follow cycling of individual particles of the archetypal cathode material, LixCoO2, within an electrode matrix.
Posted Content
Adaptive dynamic range shift (ADRIFT) quantitative phase imaging
TL;DR: This work proposes and demonstrates supersensitive QPI with an expanded dynamic range, enabled by adaptive dynamic range shift through a combination of wavefront shaping and dark-field QPI techniques, and shows dynamic range expansion (sensitivity improvement) of QPI by a factor of 6.6.
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Particle tracking of nanoparticles in soft matter
TL;DR: In this article, the authors provide a basic understanding of particle tracking instrumentation, the fundamentals of tracking analysis, and potential sources of error and bias inherent in analyzing particle tracking, as well as a brief outlook for the future particle tracking through the lens of machine learning.
References
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Journal ArticleDOI
High-speed nanoscopic tracking of the position and orientation of a single virus.
TL;DR: A colocalization methodology that combines scattering interferometry and single-molecule fluorescence microscopy to visualize both position and orientation of single quantum dot–labeled Simian virus 40 (SV40) particles suggests recurrent swap of receptors and viral pentamers as well as receptor aggregation in nanodomains.
Patent
Systems and methods for phase measurements
Christopher Fang-Yen,Gabriel Popescu,Changhuei Yang,Adam Wax,Ramachandra R. Dasari,Michael S. Feld +5 more
TL;DR: In this article, the authors present a system for phase measurement and imaging based on the principles of low coherence interferometry (LCI) using optical interferometers to measure phase, or light scattering spectroscopy (LSS) using interference within the cellular components themselves.
Journal ArticleDOI
Interferometric optical detection and tracking of very small gold nanoparticles at a water-glass interface
TL;DR: An interferometric detection scheme is used to directly detect single gold nanoparticles with a diameter as small as 5 nm in an aqueous environment and it is shown thatgold nanoparticles can be readily distinguished from background scatterers by exploiting the wavelength dependence of their plasmon resonances.
Journal ArticleDOI
Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging
TL;DR: A protocol for constructing an iSCAT microscope from commercially available optical components is presented and its compatibility with simultaneously operating single-molecule, objective-type, total internal reflection fluorescence microscopy is demonstrated.
Patent
Methods and apparatus for determining particle characteristics by measuring scattered light
TL;DR: An instrument for measuring the size and characteristics of a particle contained in a sample of particles is described in this article. But this instrument is limited to the case where the particle sample is subjected to centrifugal forces so that large particles travel in the sample chamber at velocities greater than small particles.
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