Journal ArticleDOI
High-speed nanoscopic tracking of the position and orientation of a single virus.
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TLDR
A colocalization methodology that combines scattering interferometry and single-molecule fluorescence microscopy to visualize both position and orientation of single quantum dot–labeled Simian virus 40 (SV40) particles suggests recurrent swap of receptors and viral pentamers as well as receptor aggregation in nanodomains.Abstract:
A combination of scattering interferometry and single-molecule fluorescence microscopy allows visualization of both the position and orientation of single Simian virus 40 particles on lipid bilayers and provides evidence of viral interaction with receptors in membrane nanodomains. Optical studies have revealed that, after binding, virions move laterally on the plasma membrane, but the complexity of the cellular environment and the drawbacks of fluorescence microscopy have prevented access to the molecular dynamics of early virus-host couplings, which are important for cell infection. Here we present a colocalization methodology that combines scattering interferometry and single-molecule fluorescence microscopy to visualize both position and orientation of single quantum dot–labeled Simian virus 40 (SV40) particles. By achieving nanometer spatial and 8 ms temporal resolution, we observed sliding and tumbling motions during rapid lateral diffusion on supported lipid bilayers, and repeated back and forth rocking between nanoscopic regions separated by 9 nm. Our findings suggest recurrent swap of receptors and viral pentamers as well as receptor aggregation in nanodomains. We discuss the prospects of our technique for studying virus-membrane interactions and for resolving nanoscopic dynamics of individual biological nano-objects.read more
Citations
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Journal ArticleDOI
Virus entry by endocytosis.
TL;DR: This review focuses on the cell biology of virus entry and the different strategies and endocytic mechanisms used by animal viruses.
Journal ArticleDOI
Quantum measurement and orientation tracking of fluorescent nanodiamonds inside living cells
Liam P. McGuinness,Yan Yan,Alastair Stacey,David Simpson,Liam T. Hall,Dougal Maclaurin,Steven Prawer,Paul Mulvaney,Jörg Wrachtrup,Frank Caruso,Robert E. Scholten,Lloyd C. L. Hollenberg +11 more
TL;DR: The experiments reported here demonstrate the viability of controlled single spin probes for nanomagnetometry in biological systems, opening up a host of new possibilities for quantum-based imaging in the life sciences.
Journal ArticleDOI
A review of progress in single particle tracking: from methods to biophysical insights.
TL;DR: The foundations of SPT are described together with novel optical implementations that nowadays allow the investigation of single molecule dynamic events with increasingly high spatiotemporal resolution using molecular densities closer to physiological expression levels.
Journal ArticleDOI
Quantitative mass imaging of single biological macromolecules
Gavin Young,Nikolas Hundt,Daniel Cole,Adam Fineberg,Joanna Andrecka,Andrew Tyler,Anna Olerinyova,A Ansari,Erik G. Marklund,Miranda P. Collier,Shane A. Chandler,Olga Tkachenko,Joel D. Allen,Max Crispin,Neil Billington,Yasuharu Takagi,James R. Sellers,Cédric Eichmann,Philipp Selenko,Lukas Frey,Roland Riek,Roland Riek,Martin R. Galpin,Weston B. Struwe,Justin L. P. Benesch,Philipp Kukura +25 more
TL;DR: Interferometric scattering mass spectrometry allows spatiotemporally resolved measurement of a broad range of biomolecular interactions, one molecule at a time, to characterize the molecular dynamics of processes as diverse as glycoprotein cross-linking, amyloidogenic protein aggregation, and actin polymerization.
Journal ArticleDOI
GM1 structure determines SV40-induced membrane invagination and infection
Helge Ewers,Winfried Römer,Winfried Römer,Alicia E. Smith,Kirsten Bacia,Kirsten Bacia,Serge Dmitrieff,Wengang Chai,Roberta Mancini,Jürgen Kartenbeck,Jürgen Kartenbeck,Valérie Chambon,Valérie Chambon,Ludwig Berland,Ludwig Berland,Ariella Oppenheim,Günter Schwarzmann,Ten Feizi,Petra Schwille,Pierre Sens,Ari Helenius,Ludger Johannes,Ludger Johannes +22 more
TL;DR: This analysis indicates that SV40, other polyoma viruses and some bacterial toxins use glycosphingolipids and a common pentameric protein scaffold to induce plasma membrane curvature, thus directly promoting their endocytic uptake into cells.
References
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