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Label-free microfluidic stem cell isolation technologies
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TLDR
Recent emerging label-free microfluidic based cell isolation technologies that provide unbiased stem cell enrichment based on their size and deformability, adhesion, and electrical characteristics are reviewed and discussed, and their potential to achieve effective stem cell isolation for research and clinical applications is discussed.Abstract:
Stem cell research has great potential in clinical applications and regenerative medicine, which highlight the need for efficient stem cell isolation technologies. Enrichment of stem cells is challenging because of its sensitivity to the surrounding environment and its overlapping features with non-target cells. Current gold-standard isolation techniques depend on surface antigens that are exclusively expressed on stem cells, where surface immuno-labeling is followed by flow cytometry or magnetic sorting procedures. It is well-established that stem cells should be enriched with minimal alteration, therefore, isolation without immunological tagging in a label-free format is of great interest. Microfluidic technologies have demonstrated high level of control to precisely manipulate stem cells. Here, we review the recent emerging label-free microfluidic based cell isolation technologies that provide unbiased stem cell enrichment based on their size and deformability, adhesion, and electrical characteristics, and discuss their potential to achieve effective stem cell isolation for research and clinical applications.read more
Citations
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References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
High gradient magnetic cell separation with MACS
TL;DR: A flexible, fast and simple magnetic cell sorting system for separation of large numbers of cells according to specific cell surface markers was developed and tested and makes this system an ideal complement to flow cytometry.
Journal ArticleDOI
Derivation of pluripotent stem cells from cultured human primordial germ cells
Michael J. Shamblott,Joyce Axelman,Shunping Wang,Elizabeth M. Bugg,John W. Littlefield,Peter J. Donovan,Paul D. Blumenthal,George R. Huggins,John D. Gearhart +8 more
TL;DR: In this article, a set of primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor (HILI) and forskolin.
Journal ArticleDOI
Continuous inertial focusing, ordering, and separation of particles in microchannels
TL;DR: The ability to differentially order particles of different sizes, continuously, at high rates, and without external forces in microchannels is expected to have a broad range of applications in continuous bioparticle separation, high-throughput cytometry, and large-scale filtration systems.
Journal ArticleDOI
Mesenchymal Stem Cell Homing: The Devil Is in the Details
TL;DR: A critical analysis of the methods used to track homing of exogenously infused MSCs is provided and strategies for enhancing their trafficking to particular tissues are discussed.
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