Low‐diversity bacterial community in the gut of the fruitfly Drosophila melanogaster
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TLDR
A developmental change in the most abundant species, from L. fructivorans in young adults to A. pomorum in aged adults was identified; changes in gut oxygen tension or immune system function might account for this effect.Abstract:
The bacteria in the fruitfly Drosophila melanogaster of different life stages was quantified by 454 pyrosequencing of 16S rRNA gene amplicons. The sequence reads were dominated by 5 operational taxonomic units (OTUs) at ≤ 97% sequence identity that could be assigned to Acetobacter pomorum, A. tropicalis, Lactobacillus brevis, L. fructivorans and L. plantarum. The saturated rarefaction curves and species richness indices indicated that the sampling (85,000-159,000 reads per sample) was comprehensive. Parallel diagnostic PCR assays revealed only minor variation in the complement of the five bacterial species across individual insects and three D. melanogaster strains. Other gut-associated bacteria included 6 OTUs with low %ID to previously reported sequences, raising the possibility that they represent novel taxa within the genera Acetobacter and Lactobacillus. A developmental change in the most abundant species, from L. fructivorans in young adults to A. pomorum in aged adults was identified; changes in gut oxygen tension or immune system function might account for this effect. Host immune responses and disturbance may also contribute to the low bacterial diversity in the Drosophila gut habitat.read more
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The gut microbiota of insects - diversity in structure and function.
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A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae.
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TL;DR: It is shown that the pyrroloquinoline quinone–dependent alcohol dehydrogenase activity of a commensal bacterium, Acetobacter pomorum, modulates insulin/insulin-like growth factor signaling in Drosophila to regulate host homeostatic programs controlling developmental rate, body size, energy metabolism, and intestinal stem cell activity.
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Immunity in Drosophila melanogaster — from microbial recognition to whole-organism physiology
TL;DR: The fruit fly Drosophila melanogaster has been used to understand how the immune response is regulated and coordinated at the level of the whole organism, and recent studies that have explored the effects of organism-wide regulation of theimmune response and, conversely, the effects on organism physiology are highlighted.
Drosophila microbiome modulates host developmental and metabolic homeostasis via insulin signaling
Seung Chul Shin,Seung Chul Shin,Sung-Hee Kim,Hyejin You,Hyejin You,Bo-Ram Kim,Bo-Ram Kim,Aeri C. Kim,Aeri C. Kim,Kyung-Ah Lee,Joo-Heon Yoon,Ji-Hwan Ryu,Won-Jae Lee +12 more
TL;DR: In this article, the pyrroloquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) activity of a commensal bacterium, Acetobacter pomorum, modulates insulin/insulin-like growth factor signaling (IIS) in Drosophila to regulate host homeostatic programs controlling developmental rate, body size, energy metabolism, and intestinal stem cell activity.
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