Metacaspase activity of Arabidopsis thaliana is regulated by S-nitrosylation of a critical cysteine residue.
Beatrice Belenghi,María C. Romero-Puertas,Dominique Vercammen,Anouk Brackenier,Dirk Inzé,Massimo Delledonne,Frank Van Breusegem +6 more
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TLDR
S-nitrosylation plays a central role in the regulation of the proteolytic activity of Arabidopsis thaliana metacaspase 9 (AtMC9) and it is found that AtMC9 zymogens are S-Nitrosylated at their active site cysteines in vivo and that this posttranslational modification suppresses both At MC9 autoprocessing and proteolytics activity.About:
This article is published in Journal of Biological Chemistry.The article was published on 2007-01-12 and is currently open access. It has received 242 citations till now. The article focuses on the topics: Metacaspase & Caspase.read more
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New Insights into Nitric Oxide Signaling in Plants
TL;DR: This review summarizes the basic concepts of NO signaling in animals and discusses new insights into NO enzymatic sources and molecular signaling in plants.
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Nitric oxide, stomatal closure, and abiotic stress
Steven J. Neill,Raimundo Santos Barros,Jo Bright,Radhika Desikan,John T. Hancock,Judith Harrison,Peter Morris,Dimas M. Ribeiro,Ian D. Wilson +8 more
TL;DR: The data suggest an emerging model of stress responses in which ABA has several ameliorative functions, including the rapid induction of stomatal closure to reduce transpirational water loss and the activation of antioxidant defences to combat oxidative stress.
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The hypersensitive response; the centenary is upon us but how much do we know?
TL;DR: It is considered if the HR form of cell death may occur through metabolic dysfunction in which malfunctioning organelles may play a major role and it may be better to consider the HR to be a distinctive form of plant cell death.
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Nitric oxide synthesis and signalling in plants.
TL;DR: The present understanding of how NO is produced in plants, how it is removed when its signal is no longer required and how it may be both perceived and acted upon is reviewed.
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Nitric oxide as a bioactive signalling molecule in plant stress responses
TL;DR: This review is focused mainly on the essential role of NO in plant signalling network, leading to the expression of defence response genes under various stress conditions and its ability to directly interact with other molecules and signals.
References
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Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
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Comparative Protein Modelling by Satisfaction of Spatial Restraints
Andrej Sali,Tom L. Blundell +1 more
TL;DR: A comparative protein modelling method designed to find the most probable structure for a sequence given its alignment with related structures, which is automated and illustrated by the modelling of trypsin from two other serine proteinases.
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Multiple sequence alignment with the Clustal series of programs
Ramu Chenna,Hideaki Sugawara,Tadashi Koike,Rodrigo Lopez,Toby J. Gibson,Desmond G. Higgins,Julie D. Thompson +6 more
TL;DR: The Clustal series of programs, widely used in molecular biology for the multiple alignment of both nucleic acid and protein sequences and for preparing phylogenetic trees, are extended.
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GATEWAY vectors for Agrobacterium-mediated plant transformation.
TL;DR: The GATEWAY conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids for transformation of a wide range of plant species.