Microinjection of a rabbit beta-globin gene into zygotes and its subsequent expression in adult mice and their offspring.
Thomas Wagner,Peter Hoppe,Joseph D. Jollick,David R. Scholl,Richard L. Hodinka,Janice B. Gault +5 more
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TLDR
A gene coding for rabbit beta-globin is transferred into the male pronucleus of mouse zygotes by direct microinjection to allow the introduction and expression of a broad range of genetic elements in mammals.Abstract:
We have transferred a gene coding for rabbit beta-globin into the male pronucleus of mouse zygotes by direct microinjection. Some of these zygotes developed into mature mice which contained this gene and appeared to be producing a rabbit globin. Evidence for the presence of the gene in these animals was provided by Southern blot hybridization analysis. Evidence for the expression of the rabbit gene in these transformed mice and their offspring was provided by hemoglobin isoelectric focusing analysis and specific serological reactivity between mouse anti-rabbit hemoglobin antiserum and a hemolysate from the mice that developed from the microinjected zygotes. The use of this zygote transformation may allow the introduction and expression of a broad range of genetic elements in mammals.read more
Citations
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Genetic Transformation of Drosophila with Transposable Element Vectors
TL;DR: A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene.
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Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines
TL;DR: The results of blastocyst injection studies using three independently isolated XY embryo-derived cell lines, which produce a very high proportion of live-born animals that are overtly chimaeric, are reported.
Journal ArticleDOI
Dramatic growth of mice that develop from eggs microinjected with metallothionein–growth hormone fusion genes
Richard D. Palmiter,Ralph L. Brinster,Robert E. Hammer,Myrna E. Trumbauer,Michael G. Rosenfeld,Neal C. Birnberg,Ronald M. Evans +6 more
TL;DR: A DNA fragment containing the promoter of the mouse metallothionein-I gene fused to the structural gene of rat growth hormone was microinjected into the pronuclei of fertilized mouse eggs, and seven mice developed that carried the fusion gene and six of these grew significantly larger than their littermates.
Journal ArticleDOI
Production of transgenic rabbits, sheep and pigs by microinjection
Robert E. Hammer,Vernon G. Pursel,Caird E. Rexroad,Robert J. Wall,D. J. Bolt,Karl M. Ebert,Richard D. Palmiter,Ralph L. Brinster +7 more
TL;DR: Integration of the fusion gene in all three species and expression of the gene in transgenic rabbits and pigs are reported here.
References
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Genetic transformation of mouse embryos by microinjection of purified DNA.
TL;DR: The results demonstrate that genes can be introduced into the mouse genome by direct insertion into the nuclei of early embryos by microinjected into pronuclei of fertilized mouse oocytes.
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Transformation of mammalian cells with genes from procaryotes and eucaryotes
Michael Wigler,Raymond W. Sweet,Gek Kee Sim,Barbara Wold,A. Pellicer,Elizabeth Lacy,Tom Maniatis,Saul Silverstein,Richard Axel +8 more
TL;DR: This co-transformation system should allow the introduction and stable integration of virtually any defined gene into cultured cells and is likely to represent a subpopulation of competent cells which are likely to integrate other unlinked genes at frequencies higher than the general population.
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DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells
TL;DR: The feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase to the aprt+ phenotype by means of DNA-mediated gene transfer is demonstrated.
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Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor
TL;DR: High molecular weight DNA from cells containing only one copy of the HSV gene coding for tk was successfully used to transform L+K-cells to the tk+ phenotype, and the acquired phenotype was demonstrated to be donor-derived by analysis of the electrophoretic mobility of the tK activity.