Modeling Physiological Events in 2D vs. 3D Cell Culture.
Kayla Duval,Hannah Grover,Li-Hsin Han,Yongchao Mou,Adrian F. Pegoraro,Jeffery J. Fredberg,Zi Chen +6 more
TLDR
2D and 3D cell culture methods are reviewed, advantages and limitations of these techniques in modeling physiologically and pathologically relevant processes are discussed, and directions for future research are suggested.Abstract:
Cell culture has become an indispensable tool to help uncover fundamental biophysical and biomolecular mechanisms by which cells assemble into tissues and organs, how these tissues function, and how that function becomes disrupted in disease. Cell culture is now widely used in biomedical research, tissue engineering, regenerative medicine, and industrial practices. Although flat, two-dimensional (2D) cell culture has predominated, recent research has shifted toward culture using three-dimensional (3D) structures, and more realistic biochemical and biomechanical microenvironments. Nevertheless, in 3D cell culture, many challenges remain, including the tissue-tissue interface, the mechanical microenvironment, and the spatiotemporal distributions of oxygen, nutrients, and metabolic wastes. Here, we review 2D and 3D cell culture methods, discuss advantages and limitations of these techniques in modeling physiologically and pathologically relevant processes, and suggest directions for future research.read more
Citations
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Mechanisms of 3D cell migration.
Kenneth M. Yamada,Michael Sixt +1 more
TL;DR: This Review examines how cells use both classical and novel mechanisms of locomotion as they traverse challenging 3D matrices and cellular environments and draws comparisons between 1D, 2D and 3D migration.
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Conductive biomaterials for muscle tissue engineering
TL;DR: The recent synthesis and fabrication methods of conductive scaffolds containing conductive polymers, carbon-based nanomaterials, and metal-based biomaterials were systematically discussed, and their application in a variety of forms for different kinds of muscle tissues formation were summarized.
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A Novel 3D In Vitro Platform for Pre-Clinical Investigations in Drug Testing, Gene Therapy, and Immuno-oncology
Olivia Candini,Giulia Grisendi,Elisabetta Manuela Foppiani,Matteo Brogli,Beatrice Aramini,Valentina Masciale,Carlotta Spano,Tiziana Petrachi,Elena Veronesi,Pierfranco Conte,Giorgio Mari,Massimo Dominici +11 more
TL;DR: A flat, handheld and versatile 3D cell culture bioreactor that can be loaded with tumor and/or normal cells in combination which can be monitored using a variety of read-outs, representing a valid tool useful for cancer investigations, drug screenings, and other toxicology approaches.
Journal ArticleDOI
3D-Bioprinted Mini-Brain: A Glioblastoma Model to Study Cellular Interactions and Therapeutics
Marcel A. Heinrich,Ruchi Bansal,Twan Lammers,Yu Shrike Zhang,Raymond M. Schiffelers,Jai Prakash +5 more
TL;DR: Novel 3D‐bioprinted mini‐brains consisting of glioblastoma cells and macrophages are presented as tool to study the interactions between these two cell types and to test therapeutics that target this interaction.
Journal ArticleDOI
3D In Vitro Model (R)evolution: Unveiling Tumor–Stroma Interactions
João Rodrigues,João Rodrigues,Marcel A. Heinrich,Liliana Moreira Teixeira,Liliana Moreira Teixeira,Jai Prakash +5 more
TL;DR: An overview of state-of-the-art 3D models for studying tumor-stroma interactions, with a focus on understanding why the TME is a key target in cancer therapy.
References
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