Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat.
Lígia O. Martins,Lígia O. Martins,Cláudio M. Soares,Manuela M. Pereira,Miguel Teixeira,Teresa Costa,George H. Jones,Adriano O. Henriques +7 more
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TLDR
This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3), and shows a half-life of inactivation at 80 °C of about 4 and 2 h, indicating that CotA is intrinsically highly thermostable.About:
This article is published in Journal of Biological Chemistry.The article was published on 2002-05-24 and is currently open access. It has received 502 citations till now. The article focuses on the topics: Endospore coat & Multicopper oxidase.read more
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Protein engineering of cota laccase by using bacillus subtilis spore display
TL;DR: CotA is used as a model to prove that enzymes could be improved for pH resistance by using Bacillus subtilis spore display and the t1/2 and catalytic efficiency follow the same pattern.
Development of protein display systems and genetic tools for spore-forming bacteria
TL;DR: This work aims to develop thermostable genetic reporters for Geobacilli species and to display an enzyme on the Bacillus subtilis spore surface to enhance its robustness and present an alternative to purified enzymes for industrial applications.
Dissertation
Investigating the lignocellulolytic gut microbiome of huhu grubs
TL;DR: It is suggested that the manuscript should be rewritten in a chapters-by- chapters format to facilitate more detailed discussion of the author’s motivations and methods.
Protein engineering of laccases towards higher pH
TL;DR: This document summarizes current capabilities, research and operational priorities, and plans for further studies that were established at the 2015 USGS workshop on quantitative hazard assessments of earthquake-triggered landsliding and liquefaction in the Czech Republic.
CLONING AND EXPRESSION OF LACCASE GENE PRODUCED FROM Bacillus subtilis ZHR IN E. coli
TL;DR: Ten isolates of Laccase producing bacteria were obtained from 50 isolates belongs to Bacillus sp.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
PROCHECK: a program to check the stereochemical quality of protein structures
TL;DR: The PROCHECK suite of programs as mentioned in this paper provides a detailed check on the stereochemistry of a protein structure and provides an assessment of the overall quality of the structure as compared with well refined structures of the same resolution.
Journal ArticleDOI
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
TL;DR: The MOLSCRIPT program as discussed by the authors produces plots of protein structures using several different kinds of representations, including simple wire models, ball-and-stick models, CPK models and text labels.
Journal ArticleDOI
Comparative Protein Modelling by Satisfaction of Spatial Restraints
Andrej Sali,Tom L. Blundell +1 more
TL;DR: A comparative protein modelling method designed to find the most probable structure for a sequence given its alignment with related structures, which is automated and illustrated by the modelling of trypsin from two other serine proteinases.
Book ChapterDOI
Raster3D: photorealistic molecular graphics.
Ethan A. Merritt,David Bacon +1 more
TL;DR: Raster3D is discussed, which is a suite of programs for molecular graphics, which must compromise the quality of rendered images to achieve rendering speeds high enough for useful interactive manipulation of three-dimensional objects.