Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat.
Lígia O. Martins,Lígia O. Martins,Cláudio M. Soares,Manuela M. Pereira,Miguel Teixeira,Teresa Costa,George H. Jones,Adriano O. Henriques +7 more
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TLDR
This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3), and shows a half-life of inactivation at 80 °C of about 4 and 2 h, indicating that CotA is intrinsically highly thermostable.About:
This article is published in Journal of Biological Chemistry.The article was published on 2002-05-24 and is currently open access. It has received 502 citations till now. The article focuses on the topics: Endospore coat & Multicopper oxidase.read more
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A hyperthermophilic laccase from Thermus thermophilus HB27
TL;DR: The enzyme was most notable for its striking thermophilicity; the optimal reaction temperature was ~92°C and the half-life of thermal inactivation at 80°C was >14 h, ranking it as the most thermophilic laccase reported thus far.
Journal ArticleDOI
Comparison of physico-chemical characteristics of four laccases from different basidiomycetes.
Sergey Shleev,Olga Morozova,O. V. Nikitina,E. S. Gorshina,T. V. Rusinova,Vladimir A. Serezhenkov,Dosymzhan Sh. Burbaev,Irina G. Gazaryan,Alexander I. Yaropolov +8 more
TL;DR: It could be concluded that T. hirsuta and Cerrena maxima laccases have some superior characteristics such as high stability, high activity, and low carbohydrate content, making them attractive objects for further investigations as well as for application in different areas of biotechnology.
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Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme
Laura-Leena Kiiskinen,Kristiina Kruus,Michael Bailey,Erkko Ylösmäki,Matti Siika-aho,Markku Saloheimo +5 more
TL;DR: The recombinant and wild-type laccases were compared with regard to substrate kinetics, molecular mass, pH optimum, thermostability, and processing of the N- and C-termini, and they showed very similar properties.
Journal ArticleDOI
Proteomic Analysis of the Spore Coats of Bacillus subtilis and Bacillus anthracis
Erh-Min Lai,Nikhil D. Phadke,Maureen Kachman,Rebecca Giorno,Santiago Vazquez,Jenny A. Vazquez,Janine R. Maddock,Adam Driks +7 more
TL;DR: A preliminary proteomic analysis of Bacillus subtilis and Bacillus anthracis spores suggests that a core group of coat protein species is shared between these organisms, including the major morphogenetic proteins.
Journal ArticleDOI
Thermotolerant and thermostable laccases.
TL;DR: There is a need for laccases with improved biochemical properties, such as thermostability and thermotolerance, to facilitate novel and more efficient bio-catalytic process applications.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
PROCHECK: a program to check the stereochemical quality of protein structures
TL;DR: The PROCHECK suite of programs as mentioned in this paper provides a detailed check on the stereochemistry of a protein structure and provides an assessment of the overall quality of the structure as compared with well refined structures of the same resolution.
Journal ArticleDOI
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
TL;DR: The MOLSCRIPT program as discussed by the authors produces plots of protein structures using several different kinds of representations, including simple wire models, ball-and-stick models, CPK models and text labels.
Journal ArticleDOI
Comparative Protein Modelling by Satisfaction of Spatial Restraints
Andrej Sali,Tom L. Blundell +1 more
TL;DR: A comparative protein modelling method designed to find the most probable structure for a sequence given its alignment with related structures, which is automated and illustrated by the modelling of trypsin from two other serine proteinases.
Book ChapterDOI
Raster3D: photorealistic molecular graphics.
Ethan A. Merritt,David Bacon +1 more
TL;DR: Raster3D is discussed, which is a suite of programs for molecular graphics, which must compromise the quality of rendered images to achieve rendering speeds high enough for useful interactive manipulation of three-dimensional objects.