Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat.
Lígia O. Martins,Lígia O. Martins,Cláudio M. Soares,Manuela M. Pereira,Miguel Teixeira,Teresa Costa,George H. Jones,Adriano O. Henriques +7 more
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TLDR
This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3), and shows a half-life of inactivation at 80 °C of about 4 and 2 h, indicating that CotA is intrinsically highly thermostable.About:
This article is published in Journal of Biological Chemistry.The article was published on 2002-05-24 and is currently open access. It has received 502 citations till now. The article focuses on the topics: Endospore coat & Multicopper oxidase.read more
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Structural Properties, Genomic Distribution of Laccases from Streptomyces and Their Potential Applications
TL;DR: In this article , an attempt has been made to understand the genomic distribution, complex homotrimeric structural organisation, genetic manipulations to engineer laccases with high redox potential has been discussed which can be used for several commercial applications.
Journal ArticleDOI
Wasteful Azo Dyes as a Source of Biologically Active Building Blocks
Ana C. Fernandes,Bruna Pinto,Bruna Pinto,Lorenzo Bonardo,Beatriz Royo,M. Paula Robalo,M. Paula Robalo,Lígia O. Martins +7 more
TL;DR: In this paper, an environmental-friendly enzymatic strategy was developed for the valorisation of dye-containing wastewaters, using purified preparations of enzymes for the conversion of azo dyes representative of the main classes used in the textile industry into valuable aromatic compounds: aromatic amines, phenoxinones, phenazines and naphthoquinones.
Journal ArticleDOI
An Overview of Production and Industrial Exploitation of Bacterial Laccases
TL;DR: Laccases (p-diphenol: oxygen oxidoreductase, EC 1.10.3) are multi-copper polyphenolic oxidases that oxidize many phenolic and non-phenolic aromatic compounds such as amino or methoxy monophenols as mentioned in this paper.
Book ChapterDOI
The Role of Caspases in Apoptosis and Their Inhibition in Mammalian Cell Culture
T. M. Sauerwald,M. J. Betenbaugh +1 more
TL;DR: If these approaches to manipulate caspase activity in cell lines are applied in conjunction with improvements in bioreactor design, culture medium, cell line development, and expression vector design, optimum biopharmaceutical yield will be possible.
Journal ArticleDOI
A review of microbial laccase production and activity toward different biotechnological applications
TL;DR: The potential of immobilized laccase nanoparticles for dye decolorization in microbial fuel cells has recently been studied as discussed by the authors , which can be used as biocatalyst in the bioremediation of pollutants.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
PROCHECK: a program to check the stereochemical quality of protein structures
TL;DR: The PROCHECK suite of programs as mentioned in this paper provides a detailed check on the stereochemistry of a protein structure and provides an assessment of the overall quality of the structure as compared with well refined structures of the same resolution.
Journal ArticleDOI
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
TL;DR: The MOLSCRIPT program as discussed by the authors produces plots of protein structures using several different kinds of representations, including simple wire models, ball-and-stick models, CPK models and text labels.
Journal ArticleDOI
Comparative Protein Modelling by Satisfaction of Spatial Restraints
Andrej Sali,Tom L. Blundell +1 more
TL;DR: A comparative protein modelling method designed to find the most probable structure for a sequence given its alignment with related structures, which is automated and illustrated by the modelling of trypsin from two other serine proteinases.
Book ChapterDOI
Raster3D: photorealistic molecular graphics.
Ethan A. Merritt,David Bacon +1 more
TL;DR: Raster3D is discussed, which is a suite of programs for molecular graphics, which must compromise the quality of rendered images to achieve rendering speeds high enough for useful interactive manipulation of three-dimensional objects.