Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat.
Lígia O. Martins,Lígia O. Martins,Cláudio M. Soares,Manuela M. Pereira,Miguel Teixeira,Teresa Costa,George H. Jones,Adriano O. Henriques +7 more
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TLDR
This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3), and shows a half-life of inactivation at 80 °C of about 4 and 2 h, indicating that CotA is intrinsically highly thermostable.About:
This article is published in Journal of Biological Chemistry.The article was published on 2002-05-24 and is currently open access. It has received 502 citations till now. The article focuses on the topics: Endospore coat & Multicopper oxidase.read more
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Chemical and thermal stabilization of CotA laccase via a novel one-step expression and immobilization in muNS-Mi nanospheres.
Tomás Pose-Boirazian,Gemma Eibes,Natalia Barreiro-Piñeiro,Cristina Díaz-Jullien,Juan M. Lema,José Martínez-Costas +5 more
TL;DR: A methodology that programs eukaryotic or bacterial cells to encapsulate proteins of any kind inside micro/nanospheres formed by muNS-Mi viral protein was developed in this paper.
Journal ArticleDOI
Biochemical characterization of a thermostable cobalt- or copper-dependent polyphenol oxidase with dye decolorizing ability from Geobacillus sp. JS12.
TL;DR: This is the first report of a novel thermostable polyphenol oxidase that shows the cobalt-dependent laccase activity and dye decolorization ability.
Journal ArticleDOI
Impact of orientation and flexibility of peptide linkers on T. maritima lipase Tm1350 displayed on Bacillus subtilis spores surface using CotB as fusion partner
Jawad Ullah,Huayou Chen,Huayou Chen,Åke Västermark,Jinru Jia,Bangguo Wu,Zhong Ni,Yilin Le,Hongcheng Wang +8 more
TL;DR: The activity of the displayed enzyme can be improved by maintaining orientation and flexibility of peptide linkers, to evaluate high activity and stability in industrial processes.
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A novel laccase with urate oxidation activity from Lysobacter sp. T-15.
Hideyuki Tamaki,Takeshi Matsuoka,Yuko Yasuda,Satoshi Hanada,Yoichi Kamagata,Kazunori Nakamura,Shin-ichi Sakasegawa +6 more
TL;DR: A unique urate-oxidizing enzyme was identified in a bacterium, strain T-15, and it was deemed to be a member of laccase from its broad substrate specificity, however, typical lacc enzyme and other multi-copper oxidases such as bilirubin oxidase and ascorbate oxidase seldom exhibit urate oxidation activity.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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PROCHECK: a program to check the stereochemical quality of protein structures
TL;DR: The PROCHECK suite of programs as mentioned in this paper provides a detailed check on the stereochemistry of a protein structure and provides an assessment of the overall quality of the structure as compared with well refined structures of the same resolution.
Journal ArticleDOI
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
TL;DR: The MOLSCRIPT program as discussed by the authors produces plots of protein structures using several different kinds of representations, including simple wire models, ball-and-stick models, CPK models and text labels.
Journal ArticleDOI
Comparative Protein Modelling by Satisfaction of Spatial Restraints
Andrej Sali,Tom L. Blundell +1 more
TL;DR: A comparative protein modelling method designed to find the most probable structure for a sequence given its alignment with related structures, which is automated and illustrated by the modelling of trypsin from two other serine proteinases.
Book ChapterDOI
Raster3D: photorealistic molecular graphics.
Ethan A. Merritt,David Bacon +1 more
TL;DR: Raster3D is discussed, which is a suite of programs for molecular graphics, which must compromise the quality of rendered images to achieve rendering speeds high enough for useful interactive manipulation of three-dimensional objects.