Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat.
Lígia O. Martins,Lígia O. Martins,Cláudio M. Soares,Manuela M. Pereira,Miguel Teixeira,Teresa Costa,George H. Jones,Adriano O. Henriques +7 more
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TLDR
This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3), and shows a half-life of inactivation at 80 °C of about 4 and 2 h, indicating that CotA is intrinsically highly thermostable.About:
This article is published in Journal of Biological Chemistry.The article was published on 2002-05-24 and is currently open access. It has received 502 citations till now. The article focuses on the topics: Endospore coat & Multicopper oxidase.read more
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Heterologous expression, purification, and characterization of thermo- and alkali-tolerant laccase-like multicopper oxidase from Bacillus mojavensis TH309 and determination of its antibiotic removal potential
Ali Osman Adıgüzel,Serpil Könen-Adıgüzel,Sümeyye Cilmeli,Birgül Mazmanci,Erdal Yabalak,Sevde Üstün-Odabaşı,Mehmet Ali Mazmanci +6 more
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Production and Bio Application of Laccase Enzyme for Seed Germination
TL;DR: The present paper delineates about the production and bioapplication of laccase, a multi–copper containing oxidases widely distributed in fungi, higher plants and bacteria that has the capability to oxidize a wide range of toxic and environmentally problematic substrate.
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Pseudomonas Stutzeri as Biofactories for Melanin Nanoparticle Synthesis and Its Anti-Oxidative and Antibiofilm Potential Evaluation
Dayana Mathew,Sarita G. Bhat +1 more
Resistant Bacterial Spore Coats and Their Breakdown During Germination
Anne Moir,Raymond N. Allan +1 more
TL;DR: A strain with an unusually alkali-resistant spore coat was identified as Brevibacillus borstelensis, which germinated very slowly and asynchronously, making it impractical to undertake biochemical studies of spore germination or coat degradation.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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PROCHECK: a program to check the stereochemical quality of protein structures
TL;DR: The PROCHECK suite of programs as mentioned in this paper provides a detailed check on the stereochemistry of a protein structure and provides an assessment of the overall quality of the structure as compared with well refined structures of the same resolution.
Journal ArticleDOI
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
TL;DR: The MOLSCRIPT program as discussed by the authors produces plots of protein structures using several different kinds of representations, including simple wire models, ball-and-stick models, CPK models and text labels.
Journal ArticleDOI
Comparative Protein Modelling by Satisfaction of Spatial Restraints
Andrej Sali,Tom L. Blundell +1 more
TL;DR: A comparative protein modelling method designed to find the most probable structure for a sequence given its alignment with related structures, which is automated and illustrated by the modelling of trypsin from two other serine proteinases.
Book ChapterDOI
Raster3D: photorealistic molecular graphics.
Ethan A. Merritt,David Bacon +1 more
TL;DR: Raster3D is discussed, which is a suite of programs for molecular graphics, which must compromise the quality of rendered images to achieve rendering speeds high enough for useful interactive manipulation of three-dimensional objects.