Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: The phenylacetyl-CoA catabolon
Elías R. Olivera,Baltasar Miñambres,Belén García,C. Muñiz,Miguel A. Moreno,Abel Ferrández,Eduardo Díaz,J. Garcia,José M. Luengo +8 more
TLDR
This pathway constitutes the common part (core) of a complex functional unit (catabolon) integrated by several routes that catalyze the transformation of structurally related molecules into a common intermediate (phenylacetyl-CoA).Abstract:
Fourteen different genes included in a DNA fragment of 18 kb are involved in the aerobic degradation of phenylacetic acid by Pseudomonas putida U. This catabolic pathway appears to be organized in three contiguous operons that contain the following functional units: (i) a transport system, (ii) a phenylacetic acid activating enzyme, (iii) a ring-hydroxylation complex, (iv) a ring-opening protein, (v) a β-oxidation-like system, and (vi) two regulatory genes. This pathway constitutes the common part (core) of a complex functional unit (catabolon) integrated by several routes that catalyze the transformation of structurally related molecules into a common intermediate (phenylacetyl-CoA).read more
Citations
More filters
Journal ArticleDOI
Molecular Basis of Bacterial Outer Membrane Permeability Revisited
TL;DR: This review summarizes the development in the field since the previous review and begins to understand how this bilayer of the outer membrane can retard the entry of lipophilic compounds, owing to increasing knowledge about the chemistry of lipopolysaccharide from diverse organisms and the way in which lipopoly Saccharide structure is modified by environmental conditions.
Journal ArticleDOI
Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440
Karen E. Nelson,Christian Weinel,Ian T. Paulsen,Robert J. Dodson,H. Hilbert,V. A. P. Martins Dos Santos,Derrick E. Fouts,Steven R. Gill,Mihai Pop,Mark A. Holmes,Lauren M. Brinkac,Maureen J. Beanan,Robert T. DeBoy,Sean C. Daugherty,James F. Kolonay,Ramana Madupu,William C. Nelson,Owen White,Jeremy Peterson,Hoda Khouri,Ioana R. Hance,P. Chris Lee,Erik Holtzapple,David J. Scanlan,Kevin Tran,A. Moazzez,T. Utterback,M. Rizzo,K. Lee,Daniel S. Kosack,D. Moestl,H. Wedler,J. Lauber,Diana Stjepandic,Jörg D. Hoheisel,M. Straetz,Sabina Heim,C. Kiewitz,Jonathan A. Eisen,Kenneth N. Timmis,A. Düsterhöft,Burkhard Tümmler,Claire M. Fraser +42 more
TL;DR: Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes.
Journal ArticleDOI
Microbial degradation of aromatic compounds — from one strategy to four
TL;DR: These strategies are based on different ring activation mechanisms that consist of either formation of a non-aromatic ring-epoxide under oxic conditions, or reduction of the aromatic ring under anoxic conditions using one of two completely different systems.
Journal ArticleDOI
Bacterial volatiles: the smell of small organisms
TL;DR: This review describes volatiles released into the air by bacteria growing on defined media and an effort has been made to organize the compounds according to their biosynthetic origin.
Journal ArticleDOI
Prokaryotic carbonic anhydrases
Kerry S. Smith,James G. Ferry +1 more
TL;DR: Recent work has shown that carbonic anhydrase is widespread in metabolically diverse species from both the Archaea and Bacteria domains indicating that the enzyme has a more extensive and fundamental role in prokaryotic biology than previously recognized.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum
TL;DR: Small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19 can be transferred by RP4-mediated conjugation into a wide range of Gram- and Gram+ bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination.
Journal ArticleDOI
Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.
TL;DR: The cloning system described here will be particularly useful for the construction of hybrid bacteria that stably maintain inserted genes, perhaps in competitive situations, and that do not carry antibiotic resistance markers characteristic of most available cloning vectors.