Myogenic Vector Expression of Insulin-like Growth Factor I Stimulates Muscle Cell Differentiation and Myofiber Hypertrophy in Transgenic Mice *
Michael Coleman,Francesco J. DeMayo,Kuo Chang Yin,Heung Man Lee,Robert S. Geske,Chuck Montgomery,Robert J. Schwartz +6 more
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TLDR
Results indicate that sustained overexpression of IGF-I in skeletal muscle elicits myofiber hypertrophy and provides the basis for manipulation of muscle physiology utilizing skeletal α-actin-based vectors.About:
This article is published in Journal of Biological Chemistry.The article was published on 1995-05-19 and is currently open access. It has received 662 citations till now. The article focuses on the topics: Skeletal muscle & Myocyte.read more
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Cellular and Molecular Regulation of Muscle Regeneration
TL;DR: Recent evidence supports the possible contribution of adult stem cells in the muscle regeneration process and in particular, bone marrow-derived and muscle-derived stem cells contribute to new myofiber formation and to the satellite cell pool after injury.
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The IGF-1/PI3K/Akt Pathway Prevents Expression of Muscle Atrophy-Induced Ubiquitin Ligases by Inhibiting FOXO Transcription Factors
Trevor Stitt,Doreen Drujan,Brian A. Clarke,Frank J. Panaro,Yekatarina Timofeyva,William O. Kline,Michael Gonzalez,George D. Yancopoulos,David J. Glass +8 more
TL;DR: Akt is not only capable of activating prosynthetic pathways, as previously demonstrated, but is simultaneously and dominantly able to suppress catabolic pathways, allowing it to prevent glucocorticoid and denervation-induced muscle atrophy.
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Satellite Cells and the Muscle Stem Cell Niche
TL;DR: For the last half century, the advance of molecular biology, cell biology, and genetics has greatly improved the understanding of skeletal muscle biology, with focuses on functions of satellite cells and their niche during the process ofletal muscle regeneration.
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Localized Igf-1 transgene expression sustains hypertrophy and regeneration in senescent skeletal muscle
Antonio Musarò,Karl J. A. McCullagh,Angelika Christina Paul,Leslie Houghton,Gabriella Dobrowolny,Mario Molinaro,Elisabeth R. Barton,H. L. Sweeney,Nadia Rosenthal +8 more
TL;DR: A model of persistent, functional myocyte hypertrophy is generated using a tissue-restricted transgene encoding a locally acting isoform of insulin-like growth factor-1 that is expressed in skeletal muscle (mIgf-1) and suggests clinical strategies for the treatment of age or disease-related muscle frailty.
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Skeletal muscle hypertrophy and atrophy signaling pathways.
TL;DR: Recent progress in the understanding of molecular signalling, which governs skeletal muscle atrophy and hypertrophy, and the known instances of cross-regulation between the two systems are focused on.
References
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Rapid and efficient site-specific mutagenesis without phenotypic selection.
TL;DR: The high efficiency, approximately equal to 10-fold greater than that observed using current methods without enrichment procedures, is obtained by using a DNA template containing several uracil residues in place of thymine, which is applied to mutations introduced via both oligonucleotides and error-prone polymerization.
Book
Histochemistry, theoretical and applied
TL;DR: The new Pearse bids fair to become the leader, even amongst so notable a collection of books devoted entirely or largely to histochemical techniques.
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Expression of a single transfected cDNA converts fibroblasts to myoblasts.
TL;DR: In this article, the major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family, and the expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts.