Journal ArticleDOI
NaF induces early differentiation of murine bone marrow cells along the granulocytic pathway but not the monocytic or preosteoclastic pathway in vitro.
TLDR
It is suggested that NaF induces early differentiation of bone marrow hemopoietic progenitor cells along the granulocytic pathway but not the monoicytic pathway that is linked to osteoclast formation.Abstract:
The stimulatory effects of sodium fluoride (NaF) on bone formation have been explained solely, by its activation of osteoblasts. However, whether and how NaF acts on the osteoclast linearge is poorly understood. We previously found that NaF differentiates HL-60 cells to granulocytic cells. To further test this action, we have employed here primary cultures of progenitor cells derived from murine bone marrow. NaF at subtoxic concentations (<0.5 mM) significantly up-regulated activities of several intracellular enzymes (lactate dehydrogenase, β-glucuronidase, acid phosphatase), cellular reduction of nitroblue tetrazolium, and nitric oxide (NO) production; which are all accepted as general differentiation markers. NaF (<0.5 mM) also up-regulated granulocyte-specific markers (chloroacetate esterase, cell surface antigens[Mac-1, Gr-1]) but not any of the monocyte-specific markers (nonspecific esterase, cell surface antigens [F4/80, MOMA-2]). Although other general differentiation markers (phagocytosis, adhesion, appearance, nuclear:cytoplasmic ratio) were not appreciably influenced by NaF, essentially in support of our prevous data from HL-60 cells, the present findings suggest that NaF induces early differentiation of bone marrow hemopoietic progenitor cells along the granulocytic pathway but not the monoicytic pathway that is linked to osteoclast formation. Therefore, in addition to its potent stimulatory effects on osteoblastic bone formation, NaF applied to patients with osteoporosis could be expected to indirectly reduce osteoclastic bone resorption.read more
Citations
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Journal ArticleDOI
Phenotypic variation of fluoride responses between inbred strains of mice.
Dong Yan,Thomas L. Willett,Xiao Mei Gu,E. Angeles Martinez-Mier,Laura D. Sardone,Lauren McShane,Marc D. Grynpas,Eric T. Everett +7 more
TL;DR: Strain-specific responses to F were observed for DF, serum studies, ex vivo cell culture studies, and bone quality, and among the strains, there were no patterns or significant correlations between DF severity and the actions of F on bone homeostasis.
Journal ArticleDOI
Inhibitory effects of sodium pentosan polysulfate on formation and function of osteoclasts derived from canine bone marrow
TL;DR: It is suggested that NaPPS is a novel inhibitor of RANKL and M-CSF-induced CTK, MMP-9, NFATc1, c-Fos, AP-1 upregulation, OC differentiation and bone resorption which might be a beneficial for treatment of inflammatory joint diseases and other bone diseases associated with excessive bone Resorption.
Journal ArticleDOI
Antimycin A-induced cell death depends on AIF translocation through NO production and PARP activation and is not involved in ROS generation, cytochrome c release and caspase-3 activation in HL-60 cells
TL;DR: The results suggest that AA-induced cell death does not depend on respiratory inhibition and the succeeding cascades, but on NO production, PARP overactivation and AIF translocation.
Journal Article
Induction of apoptosis by sodium fluorosilicate treatment in human osteogenic sarcoma (HOS) cells.
TL;DR: Sodium fluorosilicate induces apoptosis in HOS cells through decrease in Bcl-2, the release of cytochrome c to the cytosol and activation of caspase-3.
Journal ArticleDOI
Possible protective role of grape seed proanthocyanidin on fluoride induced testicular toxicity in adult and young albino rats
TL;DR: Co-administration of NaF and GSP showed significant increase of plasma testosterone, FSH and LH levels in adult rats and decrease in serum MDA and nitrite levels compared to NaF alone or follow up group in adult and young rats.
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