Journal ArticleDOI
New polygalacturonases from Trichoderma reesei: characterization and their specificities to partially methylated and acetylated pectins.
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TLDR
Two extracellular isoenzymes of polygalacturonases PG1 and PG2 were isolated from 3-day-old culture filtrates of Trichoderma reesei and a mode of action revealed a random cleavage pattern for PG2, confirming that these enzymes are endopolygalactonases.About:
This article is published in Carbohydrate Research.The article was published on 2003-03-14. It has received 59 citations till now. The article focuses on the topics: Trichoderma reesei & Pectinase.read more
Citations
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Journal ArticleDOI
The ThPG1 Endopolygalacturonase Is Required for the Trichoderma harzianum-Plant Beneficial Interaction
Eugenia Morán-Diez,Rosa Hermosa,Patrizia Ambrosino,Rosa E. Cardoza,Santiago Gutiérrez,Matteo Lorito,Enrique Monte +6 more
TL;DR: The endoPG-encoding gene was found to be required for active root colonization and plant defense induction by T. harzianum T34, and in vivo assays showed that Botrytis cinerea leaf necrotic lesions were slightly smaller in plants colonized by ePG5, although no statistically significant differences were observed.
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Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30
TL;DR: The growth and enzyme production by Trichoderma reesei Rut C-30 using different lignocellulosic materials as carbon source were investigated and it was found that endoglucanase and endoxylanse activities were produced throughout the cultivations, whereas α-arabinosidase was induced late during the cultivation.
Journal ArticleDOI
Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2003-2004
TL;DR: This review is the third update of the original review on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings the topic to the end of 2004.
Journal ArticleDOI
Physical characterisation of the rhamnogalacturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin
Gordon A. Morris,Gordon A. Morris,Marie-Christine Ralet,Estelle Bonnin,Jean Franois Thibault,Stephen E. Harding +5 more
TL;DR: In this paper, the degradation of the HG region has an important impact on intrinsic viscosity, but less on molar mass and the inverse is true for degradation of RG-I region.
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Degradation of different pectins by fungi: correlations and contrasts between the pectinolytic enzyme sets identified in genomes and the growth on pectins of different origin
Isabelle Benoit,Pedro M. Coutinho,Henk A. Schols,Jan P. Gerlach,Bernard Henrissat,Ronald P. de Vries +5 more
TL;DR: This study highlights the complexity of the mechanisms involved in fungal degradation of complex carbon sources such as pectins and suggests mining genomes and comparative genomics are promising first steps towards the production of specific pectinolytic fractions.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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A photometric adaptation of the somogyi method for the determination of glucose
TL;DR: In this paper, the reliability of the various Somogyi-Shaffer-Hartmann (SHH) copper reagents for glucose determination in biological material has been established, which can be accomplished by omission of the iodide and iodate in their preparation, since these interfere with the molybdate color reagents.
Reference BookDOI
Food Polysaccharides and Their Applications
TL;DR: Alistair M. Stephen and Shirley C. Cui as mentioned in this paper proposed a method for the detection of polysaccharides in foods, and showed that the method can be applied in the field of agriculture.