Open Access
Next-generation in vivo optical imaging with short-wave infrared quantum dots
Lars Riedemann,Alexander Bartelt,Frank B. Jaworski,Jonas Kloepper,Joerg Heeren,Peter T. C. So,Dai Fukumura,Rakesh K. Jain,Oliver T. Bruns,Thomas S. Bischof,Daniel K. Harris,Daniel Franke,Yanxiang Shi,Jessica A. Carr,Christopher J. Rowlands,Mark W. Wilson,Ou Chen,He Wei,Gyu Weon Hwang,Daniel M Montana Fernandez,Igor Coropceanu,Odin B. Achorn,Klavs F. Jensen,Moungi G. Bawendi +23 more
TLDR
In this paper, a class of high-quality SWIR-emissive indium-arsenide-based quantum dots (QDs) are introduced for various functional imaging applications, and that exhibit narrow and size-tunable emission and a dramatically higher emission quantum yield than previously described SWIR probes.Abstract:
For in vivo imaging, the short-wavelength infrared region (SWIR; 1000–2000 nm) provides several advantages over the visible and near-infrared regions: general lack of autofluorescence, low light absorption by blood and tissue, and reduced scattering. However, the lack of versatile and functional SWIR emitters has prevented the general adoption of SWIR imaging by the biomedical research community. Here, we introduce a class of high-quality SWIR-emissive indium-arsenide-based quantum dots (QDs) that are readily modifiable for various functional imaging applications, and that exhibit narrow and size-tunable emission and a dramatically higher emission quantum yield than previously described SWIR probes. To demonstrate the unprecedented combination of deep penetration, high spatial resolution, multicolor imaging and fast-acquisition-speed afforded by the SWIR QDs, we quantified, in mice, the metabolic turnover rates of lipoproteins in several organs simultaneously and in real time as well as heartbeat and breathing rates in awake and unrestrained animals, and generated detailed three-dimensional quantitative flow maps of the mouse brain vasculature.read more
Citations
More filters
Journal ArticleDOI
Development of organic semiconducting materials for deep-tissue optical imaging, phototherapy and photoactivation
Jingchao Li,Kanyi Pu +1 more
TL;DR: This review summarizes the recent progress in the development of OSMs based on small-molecule fluorophores, aggregation-induced emission (AIE) dyes and semiconducting oligomer/polymer nanoparticles (SONs/SPNs) for advanced biophotonic applications and highlights OSMs as a multifunctional platform for a wide range of biomedical applications.
Journal ArticleDOI
Near-Infrared-II Molecular Dyes for Cancer Imaging and Surgery.
TL;DR: This review focuses on summarizing organic dyes emitting at a biological transparency window termed the near‐infrared‐II (NIR‐II) window, where minimal light interaction with the surrounding tissues allows photons to travel nearly unperturbed throughout the body.
Journal ArticleDOI
Lifetime-engineered NIR-II nanoparticles unlock multiplexed in vivo imaging.
Yong Fan,Peiyuan Wang,Yiqing Lu,Rui Wang,Lei Zhou,Xianlin Zheng,Xiaomin Li,James A. Piper,Fan Zhang +8 more
TL;DR: Lanthanide-doped NIR-II nanoparticles with engineered luminescence lifetimes are presented with in vivo quantitative imaging using time-domain multiplexing to identify tumour subtypes in living mice and it is demonstrated that robust lifetime coding is independent of tissue penetration depth.
Journal ArticleDOI
Concepts of nanoparticle cellular uptake, intracellular trafficking, and kinetics in nanomedicine.
TL;DR: An overview of the field`s understanding of how nanoparticle physicochemical properties affect cellular interactions is summarized, cellular internalization pathways are reviewed, and intracellular nanoparticle trafficking and kinetics are explored.
Journal ArticleDOI
Recent Advances of Optical Imaging in the Second Near-Infrared Window
Kenry,Yukun Duan,Bin Liu +2 more
TL;DR: The importance of biological imaging in the NIR‐II spectral region is highlighted, the emergence and latest development of various Nir‐II fluorescence and PA imaging probes and their applications are discussed, and Perspectives on the promises and challenges facing this nascent yet exciting field are given.
References
More filters
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
Fiji: an open-source platform for biological-image analysis
Johannes Schindelin,Ignacio Arganda-Carreras,Erwin Frise,Verena Kaynig,Mark Longair,Tobias Pietzsch,Stephan Preibisch,Curtis Rueden,Stephan Saalfeld,Benjamin Schmid,Jean-Yves Tinevez,Daniel J. White,Volker Hartenstein,Kevin W. Eliceiri,Pavel Tomancak,Albert Cardona +15 more
TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
Journal ArticleDOI
Particle-Imaging Techniques for Experimental Fluid Mechanics
TL;DR: A review of these methods can be found in articles by Lauterborn & Vogel (1984), Adrian (1986a), Hesselink (1988), and Dudderar et al..
Journal ArticleDOI
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
Benoit Dubertret,Paris A. Skourides,David J. Norris,David J. Norris,Vincent Noireaux,Ali H. Brivanlou,Albert Libchaber +6 more
TL;DR: C encapsulated individual nanocrystals in phospholipid block–copolymer micelles acted as in vitro fluorescent probes to hybridize to specific complementary sequences and were followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis.
Journal ArticleDOI
Core/Shell semiconductor nanocrystals.
TL;DR: The fundamental properties and synthesis methods of core/shell and core/multiple shell structures of II- VI, IV-VI, and III-V semiconductors are discussed.
Related Papers (5)
Next-generation optical imaging with short-wave infrared quantum dots.
Oliver T. Bruns,Thomas S. Bischof,Daniel K. Harris,Daniel Franke,Yanxiang Shi,Lars Riedemann,A. Bartelt,Frank B. Jaworski,Jessica A. Carr,Christopher J. Rowlands,Mark W. Wilson,Ou Chen,He Wei,Gyu Weon Hwang,Daniel M. Montana,Igor Coropceanu,Odin B. Achorn,Jonas Kloepper,Joerg Heeren,Peter T. C. So,Dai Fukumura,Klavs F. Jensen,Rakesh K. Jain,Moungi G. Bawendi +23 more